Interindividual differences of dietary fat‐inducible Mest in white adipose tissue of C57BL/6J mice are not heritable

Objective Differences in white adipose tissue (WAT) expression of mesoderm‐specific transcript (Mest) in C57BL6/J mice fed a high‐fat diet (HFD) are concomitant with and predictive for the development of obesity. However, the basis for differences in WAT Mest among mice is unknown. This study invest...

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Published in:Obesity (Silver Spring, Md.) Md.), 2024-06, Vol.32 (6), p.1144-1155
Main Authors: Anunciado‐Koza, Rea Victoria P., Yin, Haifeng, Bilodeau, Crystal L., Cooke, Diana, Ables, Gene P., Ryzhov, Sergey, Koza, Robert A.
Format: Article
Language:English
Subjects:
Adipocytes
Adipose Tissue, White - metabolism
Age
Animals
Biopsy
Body fat
Diet
Diet, High-Fat
Dietary Fats - adverse effects
Epigenetics
Female
Females
Gene expression
Laboratory animals
Lipids
Liver - metabolism
Male
Metabolites
Mice
Mice, Inbred C57BL
Obesity
Obesity - genetics
Obesity - metabolism
Phenotype
Proteins
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Summary:Objective Differences in white adipose tissue (WAT) expression of mesoderm‐specific transcript (Mest) in C57BL6/J mice fed a high‐fat diet (HFD) are concomitant with and predictive for the development of obesity. However, the basis for differences in WAT Mest among mice is unknown. This study investigated whether HFD‐inducible WAT Mest, as well as susceptibility to obesity, is transmissible from parents to offspring. Methods WAT biopsies of mice fed an HFD for 2 weeks identified parents with low and high WAT Mest for breeding. Obesity phenotypes, WAT Mest, hepatic gene expression, and serum metabolites were determined in offspring fed an HFD for 2 weeks. Results Offspring showed no heritability of obesity or WAT Mest phenotypes from parents but did show hepatic and serum metabolite changes consistent with their WAT Mest. Importantly, retired male breeders showed WAT Mest expression congruent with initial WAT biopsies even though HFD exposure occurred early in life. Conclusions Disparity of HFD‐induced Mest in mice is not heritable but, rather, is reestablished during each generation and remains fixed from an early age to adulthood. Short‐term HFD feeding reveals variation of WAT Mest expression within isogenic mice that is positively associated with the development of obesity.
ISSN:1930-7381
1930-739X
1930-739X
DOI:10.1002/oby.24020