Circulating miR-30e-3p induces disruption of neurite development in SH-SY5Y cells by targeting ABI1, a novel biomarker for schizophrenia

• Published in: Journal of psychiatric research 2024-06, Vol.174, p.84-93
• Main Authors: Jin, Mengdi, Xie, Mengtong, Liu, Yane, Song, Haideng, Zhang, Min, Li, Weizhen, Li, Xinwei, Jia, Ningning, Dong, Lin, Lu, Qingxing, Xue, Fengyu, Yan, Lijuan, Yu, Qiong
• Format: Article
• Language: English
• Subjects: ABI1; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Apoptosis - drug effects; Apoptosis - physiology; Biomarkers - blood; Biomarkers - metabolism; Cell Line, Tumor; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - metabolism; Humans; MicroRNAs - blood; MicroRNAs - genetics; miR-30e-3p; Neurite development; Neurites - drug effects; Neuroblastoma; Schizophrenia; Schizophrenia - blood; Schizophrenia - metabolism; Tretinoin - pharmacology; Tubulin - metabolism
• ISSN: 0022-3956; 1879-1379; 1879-1379
• DOI: 10.1016/j.jpsychires.2024.04.005

Schizophrenia (SCZ) represents a set of enduring mental illnesses whose underlying etiology remains elusive, posing a significant challenge to public health. Previous studies have shown that the neurodevelopmental process involving small molecules such as miRNA and mRNA is one of the etiological hypotheses of SCZ. We identified and verified that miR-30e-3p and ABI1 can be used as biomarkers in peripheral blood transcriptome sequencing data of patients with SCZ, and confirmed the regulatory relationship between them. To further explore their involvement, we employed retinoic acid (RA)-treated SH-SY5Y differentiated cells as a model system. Our findings indicate that in RA-induced SH-SY5Y cells, ABI1 expression is up-regulated, while miR-30e-3p expression is down-regulated. Functionally, both miR-30e-3p down-regulation and ABI1 up-regulation promote apoptosis and inhibit the proliferation of SH-SY5Y cells. Subsequently, the immunofluorescence assay detected the expression location and abundance of the neuron-specific protein β-tubulinIII. The expression levels of neuronal marker genes MAPT, TUBB3 and SYP were detected by RT-qPCR. We observed that these changes of miR-30e-3p and ABI1 inhibit the neurite growth of SH-SY5Y cells. Rescue experiments further support that ABI1 silencing can correct miR-30e-3p down-regulation-induced SH-SY5Y neurodevelopmental defects. Collectively, our results establish that miR-30e-3p′s regulation of neurite development in SH-SY5Y cells is mediated through ABI1, highlighting a potential mechanism in SCZ pathogenesis.