A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2

Streptococcus suis serotype 2 is an economically important zoonotic pathogen that causes septicemia, arthritis, and meningitis in pigs and humans. S. suis serotype 2 is responsible for substantial economic losses to the swine industry and poses a serious threat to public health, and accurate and rap...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2024-01, Vol.267, p.125202-125202, Article 125202
Main Authors: Wang, Lu, Sun, Jing, Zhao, Jiyu, Bai, Jieyu, Zhang, Yueling, Zhu, Yao, Zhang, Wanjiang, Wang, Chunlai, Langford, Paul R., Liu, Siguo, Li, Gang
Format: Article
Language:English
Subjects:
arthritis
Cas12a
CRISPR
cross reaction
crRNA
detection limit
Diagnostics
human resources
meningitis
pathogens
point-of-care systems
pork industry
public health
rapid methods
recombinase polymerase amplification
septicemia
serotypes
Streptococcus suis
Streptococcus suis serotype 2
swine
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Streptococcus suis serotype 2 is an economically important zoonotic pathogen that causes septicemia, arthritis, and meningitis in pigs and humans. S. suis serotype 2 is responsible for substantial economic losses to the swine industry and poses a serious threat to public health, and accurate and rapid detection is important for the prevention and control of epidemic disease. In this study, we developed a high-fidelity detection and serotyping platform for S. suis serotype 2 based on recombinase polymerase amplification (RPA) and a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a system called Cards-SSJ/K. Cards-SSJ had a detection limit of 10 CFU, takes
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2023.125202