Using structural equation modeling to assess the genotoxic and mutagenic effects of heavy metal contamination in the freshwater ecosystems: A study involving Oreochromis niloticus in an urban river

• Published in: The Science of the total environment 2024-02, Vol.913, p.169529
• Main Authors: Silva, Jordany Gomes da, Chagas, Cristiano Aparecido, Souza, Talita Giselly Dos Santos, Araújo, Marlyete Chagas de, Araújo, Lívia Caroline Alexandre de, Santos, André Maurício Melo, Sá, Rafael Artur de Queiroz Cavalcanti de, Alves, Rayanna Barroso de Oliveira, Rodrigues, Rosner Henrique Alves, Silva, Hernande Pereira da, Malafaia, Guilherme, Bezerra, Ranilson de Souza, Oliveira, Maria Betânia Melo de
• Format: Article
• Language: English
• Subjects: Animals; Brazil; cadmium; chromium; Cichlids; comet assay; DNA; DNA Damage; Ecosystem; ecotoxicology; environment; Environmental Monitoring - methods; equations; Fresh Water; freshwater; genotoxicity; heavy metals; Latent Class Analysis; manganese; Metals, Heavy - analysis; Metals, Heavy - toxicity; mutagens; Mutagens - analysis; Mutagens - toxicity; Oreochromis niloticus; pollution; rivers; Rivers - chemistry; species; structural equation modeling; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity; water quality; zinc
• ISSN: 1879-1026; 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169529

Chemical pollutants represent a leading problem for aquatic ecosystems, as they can induce genetic, biochemical, and physiological changes in the species of these ecosystems, thus compromising their adaptability and survival. The Capibaribe River runs through the state of Pernambuco, located in Northeastern Brazil, and passes through areas of agricultural cultivation, densely populated cities, and industrial centers, primarily textiles. Despite its importance, few ecotoxicological studies have been conducted on its environment, and knowledge about pollution patterns and their effects on its biota is still being determined. The objective of this study was to evaluate the water quality and the damage supposed to be caused by pollutants on the DNA specimens of Nile tilapia (Oreochromis niloticus) obtained from seven strategic points of Capibaribe. Tilapia specimens and water were collected during the rainy and dry seasons from 2015 to 2017. The following characteristics were analyzed: physicochemical (six), metal concentration (seven), local pluviosity, micronuclei, and comet assay. The physicochemical and heavy metal analyses were exploratory, whereas the ecotoxicological analyses were hypothetical. To verify this hypothesis, we compared the groups of fish collected to the results of the micronuclei test and comet assay. We created a Structural Equation Model (SEM) to determine how each metal's micronuclei variables, damage index, pluviosity, and concentration were related. Our results demonstrated that the highest values for markers of genetic damage were detected at points with the highest heavy metal concentrations, especially iron, zinc, manganese, chromium, and cadmium. The SEM demonstrated that metals could explain the findings of the genotoxicity markers. Moreover, other pollutants, such as pesticides, should be considered, mainly where the river passes through rural areas. The results presented here demonstrate that the Capibaribe River has different degrees of contamination and confirm our hypothesis.