Quantified trend of photosynthetic rate along the depth of microalgae biofilm

• Published in: The Science of the total environment 2023-06, Vol.876, p.162801-162801, Article 162801
• Main Authors: Zhuang, Lin-Lan, Tian, Wanqing, Yang, Yanan, Ge, Shuhan, Li, Peihua, Sun, Shuo, Zhang, Jian, Liang, Shuang
• Format: Article
• Language: English
• Subjects: Attached microalgae cultivation; biofilm; Biofilms; Biomass; dissolved oxygen; environment; Light; light intensity; Micro-environment of biofilm; Microalgae; Microalgae biofilm; Oxygen; Oxygen distribution; Photosynthesis; Quantitative photosynthetic rate; wastewater treatment
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.162801

Compared with suspended microalgae cultivation, attached microalgae cultivation for wastewater treatment has advantages of low biomass recovery costs and high robustness. As a heterogeneous system, the variation of photosynthetic capacity along biofilm depth lacks quantitative conclusions. The distribution curve of oxygen concentration along the depth of attached microalgae biofilm (f(x)) was detected by dissolved oxygen (DO) microelectrode, and a quantified model was built based on mass conservation and Fick's law. It revealed that the net photosynthetic rate at a certain depth (x) in the biofilm showed a linear relationship with the second derivatives of the distribution curve of oxygen concentration (f″(x)). In addition, the declining trend of photosynthetic rate along attached microalgae biofilm was relatively slow compared with the suspended system. The photosynthetic rate at 150–200 μm depth of algae biofilm was only 3.60 %–17.86 % of that at the surface layer. Moreover, the light saturation points of the attached microalgae got lower along the depth of biofilm. Compared to 400 lx light intensity, the net photosynthetic rate of microalgae biofilm at the depths of 100–150 μm and 150–200 μm increased by 389 % and 956 % under 5000 lx, respectively, showing the high photosynthesis potential with increasing light. [Display omitted] •Quantified photosynthetic rate changes along algae biofilm depth were first reported.•Light attenuation in microalgal biofilm was different from suspended cultivation.•Photosynthetic rate at 150–200 μm depth was 3.60 %–17.86 % as much as surface biofilm.•Deep layer algae could regain high photosynthesis under enhanced illuminance.•Deep layer microalgae had relatively low light saturation point.