Maternal non-compliance with recommended folic acid supplement use alters global DNA methylation in cord blood of newborns: A cohort study

Prenatal folate exposure may alter epigenetic marks in the offspring. We aimed to evaluate associations between prenatal exposure to folic acid (FA) in preconception and in utero with cord blood DNA methylation in long interspersed nuclear element 1 (LINE-1) and Alu short interspersed nuclear elemen...

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Published in:Clinical nutrition (Edinburgh, Scotland) Scotland), 2024-06, Vol.43 (6), p.1191-1198
Main Authors: Morales, Eva, Prieto-Sánchez, María Teresa, Mendiola, Jaime, Cutillas-Tolín, Ana, Adoamnei, Evdochia, Valera-Gran, Desirée, Martínez-Graciá, Carmen, Santaella-Pascual, Marina, Suárez-Martinez, Clara, Vioque, Jesús, Castaños, María Jesús, del Castillo, Eva, García-Marcos, Luis
Format: Article
Language:English
Subjects:
Alu
Cord blood
DNA methylation
Folic acid
LINE-1
Supplements
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Summary:Prenatal folate exposure may alter epigenetic marks in the offspring. We aimed to evaluate associations between prenatal exposure to folic acid (FA) in preconception and in utero with cord blood DNA methylation in long interspersed nuclear element 1 (LINE-1) and Alu short interspersed nuclear elements (SINEs) as markers of global DNA methylation levels. Data come from 325 mother–child pairs participating in the Nutrition in Early Life and Asthma (NELA) birth cohort (2015–2018). Pregnant women were asked about supplement use, including brand name and dose, one month before pregnancy (preconception) and through the trimesters of pregnancy. Maternal dietary folate intake was assessed using a validated food frequency questionnaire with additional questions for FA supplement use. Folate serum levels were measured in mothers at 24 weeks of gestation and in cord blood of newborns. DNA methylation was quantitatively assessed by bisulfite pyrosequencing on 5 LINE-1 and 3 Alu different elements. Associations were estimated using multivariable linear regression models. A reduction in methylation levels of LINE-1 in newborns was associated with the use of FA supplements below the recommended doses (
ISSN:0261-5614
1532-1983
DOI:10.1016/j.clnu.2024.04.007