Genome-wide identification of bHLH transcription factors and expression analysis under drought stress in Pseudoroegneria libanotica at germination

• Published in: Physiology and molecular biology of plants 2024-03, Vol.30 (3), p.467-481
• Main Authors: Zhai, Xingguan, Wang, Xia, Yang, Xunzhe, Huang, Qingxiang, Wu, Dandan, Wang, Yi, Kang, Houyang, Sha, Lina, Fan, Xing, Zhou, Yonghong, Zhang, Haiqin
• Format: Article
• Language: English
• Subjects: Aridity; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Cell Biology; Collinearity; Drought resistance; Genes; Genomes; Germination; Heavy metals; Helix-loop-helix proteins (basic); Homology; Life Sciences; Metabolism; Photomorphogenesis; Plant growth; Plant Physiology; Plant Sciences; Plants (botany); Pseudoroegneria; Research Article; Semi arid areas; Signal transduction; Transcription factors; Transcriptomes; Triticeae; Yeasts
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-024-01433-w

The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in Pseudoroegneria species has not been studied yet. Pseudoroegneria (Nevski) Á. Löve is a perennial genus of the Triticeae . Pseudoroegneria species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in Pseudoroegneria libanotica , which could be classified into 15 groups. Collinearity analysis indicates that 122 PlbHLH genes share homology with wbHLH genes in wheat, and it has lower homology with AtbHLH genes in Arabidopsis . Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated PlbHLH genes were screened. Among them, PlbHLH6 , PlbHLH55 and PlbHLH64 as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of PlbHLHs in Pse. libanotica , and discovered new drought-tolerance candidate genes to enhance the genetic background of Triticeae crops.