Transcriptomic and proteomic features of a mouse model of sperm DNA damage induced by benzo(a)pyrene

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2024-06, Vol.126, p.108596, Article 108596
• Main Authors: Zhang, Chenming, Ma, Yunfeng, Liu, Wenbang, Ma, Sicheng, Chen, Zhelin, Hao, XiaoHui, Sun, Zixue, Wang, Zulong
• Format: Article
• Language: English
• Subjects: Animals; Benzo(a)pyrene (BaP); Benzo(a)pyrene - toxicity; DNA Damage; DNA Fragmentation - drug effects; Male; Mice; Proteome - drug effects; Proteomics; Sperm DNA fragmentation; Spermatozoa - drug effects; Spermatozoa - metabolism; Testis - drug effects; Testis - metabolism; Testis - pathology; The PI3K-Akt signaling pathway; Transcriptome - drug effects; Transcriptomics
• ISSN: 0890-6238; 1873-1708; 1873-1708
• DOI: 10.1016/j.reprotox.2024.108596

This study replicated a mouse model of sperm DNA damage induced by benzo(a)pyrene (BaP), and the transcriptomic and proteomic features of the model were examined to clarify the pathways related to BaP-induced damage to sperm DNA. Male mice in the BaP group were subjected to BaP at a dosage of 100 mg/kg/d or an equivalent quantity of saline solution in the control group for 60 days. Subsequently, the DNA fragmentation index (DFI) in sperm was assessed using a sperm chromatin structure assay (SCSA). RNA-seq and data-independent acquisition (DIA) were used to identify the mRNA and protein expression patterns in the testis. The sperm DFI significantly increased in the BaP group. Compared to the control group, the BaP group exhibited differential expression of 240 genes (referred to as DEGs) and 616 proteins (referred to as DEPs). These molecules included Aldh1a1, Cyb5r3, Fads1, Oxsm, Rcn3, and Prss45. Pathways in cancer, the PI3K-Akt signaling pathway, metabolic pathways, and the MAPK signaling pathway were the primary areas where these genes showed enrichment. BaP can damage the DNA of sperm and affect metabolism, the PI3K-Akt pathway, and pathways associated with cancer signaling. •This study replicated a mouse model of sperm DNA damage induced by benzo(a)pyrene.•The transcriptomic and proteomic features of the model were examined.•Benzo(a)pyrene could be an important contributor to the harmful effects on sperm DNA.•Benzo(a)pyrene harms sperm DNA via metabolism, PI3K-Akt/cancer signaling pathways.