Correlations between the degree of infection by wild strain of Toxoplasma gondii in vitro and porcine hematological parameters

• Published in: Experimental parasitology 2024-06, Vol.261, p.108754, Article 108754
• Main Authors: Barros Oliveira, Carlos Vinicius, Paulino da Silva, Maria Elenilda, de Lima, Jailson Renato, Tavares Moreira, Amanda Maria, Mendes Brito, Maria Jéssica, Coelho Gonçalves, Cicera Alane, Lemos de Barros, João Eudes, de Oliveira, Romário Matheus, Kamdem, Jean Paul, Barros, Luiz Marivando, Duarte, Antonia Eliene
• Format: Article
• Language: English
• Subjects: Apicomplexa; eosinophils; Hematological; inoculum; Leukocytes; monocytes; multivariate analysis; neutrophils; Parasitemia; parasites; parasitology; peptides; Protozoa; swine; Toxoplasma gondii
• ISSN: 0014-4894; 1090-2449; 1090-2449
• DOI: 10.1016/j.exppara.2024.108754

The apicomplexa Toxoplasma gondii is capable of actively proliferating in numerous types of nucleated cells, and therefore has a high potential for dissemination and resistance. Thus, the present work aimed to correlate the inoculum concentrations and amount of post-infection parasites with porcine hematological parameters (including biochemistry) through in vitro culture. Porcine blood was incubated with different concentrations of parasites (1.2 × 107, 6/3/1.5 × 106 cells/mL), then the concentrations of red blood cells (RBC) and their morphology, total and differential leukocytes, and free peptides were evaluated. In addition, eight different blood samples analyzed before inoculation, where subsequent multivariate analysis was applied to correlate different variables with trophozoite concentration. The results showed no significant variation (p