Unraveling the Mechanisms of Efficient Phosphorus Utilization in Popcorn (Zea mays L. var. everta): Insights from Proteomic and Metabolite Analysis

• Published in: Journal of proteome research 2024-08, Vol.23 (8), p.3108-3123
• Main Authors: Barboza Bispo, Rosimeire, Pinto, Vitor Batista, de Oliveira Santos, Talles, Jário de Lima, Valter, Rohem Simão, Bruna, Fischer, Anne, Naldrett, Michael J., Alvarez, Sophie
• Format: Article
• Language: English
• Subjects: Flavonoids - metabolism; Gene Expression Regulation, Plant; Phosphorus - metabolism; Photosynthesis; Plant Proteins - genetics; Plant Proteins - metabolism; Proteome - metabolism; Proteomics - methods; Stress, Physiological; Zea mays - genetics; Zea mays - growth & development; Zea mays - metabolism
• ISSN: 1535-3893; 1535-3907; 1535-3907
• DOI: 10.1021/acs.jproteome.3c00772

The expansion of agriculture and the need for sustainable practices drives breeders to develop plant varieties better adapted to abiotic stress such as nutrient deficiency, which negatively impacts yields. Phosphorus (P) is crucial for photosynthesis and plant growth, but its availability in the soil is often limited, hampering crop development. In this study, we examined the response of two popcorn inbred lines, L80 and P7, which have been characterized previously as P-use inefficient and P-use efficient, respectively, under low (stress) and high P (control) availability. Physiological measurements, proteomic analysis, and metabolite assays were performed to unravel the physiological and molecular responses associated with the efficient use of P in popcorn. We observed significant differences in protein abundances in response to the P supply between the two inbred lines. A total of 421 differentially expressed proteins (DEPs) were observed in L80 and 436 DEPs in P7. These proteins were involved in photosynthesis, protein biosynthesis, biosynthesis of secondary metabolites, and energy metabolism. In addition, flavonoids accumulated in higher abundance in P7. Our results help us understand the major components of P utilization in popcorn, providing new insights for popcorn molecular breeding programs.