Changes on corpus luteum structure and progesterone synthesis pathway after hCG or GnRH treatment during the early luteal phase in sheep

This study investigated the effect of hCG or GnRH on structural changes of the corpora lutea (CL) and the regulation of the expression of steroidogenic enzymes involved in P4 secretion in post-ovulatory (po-CL) and accessory CL (acc-CL). Sixty-four ewes were assigned to three groups receiving: 300 I...

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Bibliographic Details
Published in:Animal reproduction science 2024-06, Vol.265, p.107474, Article 107474
Main Authors: Fernández, Jimena, Bruno-Galarraga, María Macarena, Cueto, Marcela Isabel, Bonadeo, Nadia, Notaro, Ulises, Soto, Andrés Telésforo, de la Sota, Rodolfo Luzbel, Salvetti, Natalia Raquel, Bianchi, Carolina Paula, Cristina, Carolina, Ortega, Hugo Héctor, Gibbons, Alejandro Eduardo, Lacau-Mengido, Isabel María
Format: Article
Language:English
Subjects:
Accessory corpora lutea
Animals
Cholesterol Side-Chain Cleavage Enzyme - genetics
Cholesterol Side-Chain Cleavage Enzyme - metabolism
Chorionic Gonadotropin - administration & dosage
Chorionic Gonadotropin - pharmacology
Corpus Luteum - drug effects
Corpus Luteum - metabolism
Ewes
Female
Gonadotropin-Releasing Hormone - metabolism
Gonadotropin-Releasing Hormone - pharmacology
Luteal cell
Luteal function
Luteal Phase - drug effects
Luteinizing Hormone - metabolism
Phosphoproteins
Progesterone - blood
Progesterone - metabolism
Sheep - physiology
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Summary:This study investigated the effect of hCG or GnRH on structural changes of the corpora lutea (CL) and the regulation of the expression of steroidogenic enzymes involved in P4 secretion in post-ovulatory (po-CL) and accessory CL (acc-CL). Sixty-four ewes were assigned to three groups receiving: 300 IU of hCG (hCG) or 4 µg Buserelin (GnRH) or 1 mL of saline solution (Control) on Day (d) 4 post artificial insemination (FTAI). Laparoscopic ovarian were performed on d 4, 14 and, 21 post-FTAI to determine the numbers of CL. Blood samples were collected for serum LH and P4 analysis. On d 14 post-FTAI, both CL were removed from the ovary to determine large luteal cell (LLC) number and to evaluate the expression of steroidogenic enzymes (HSD3B1, STAR, CYP11A1). Only hCG and GnRH treated ewes generated acc-CL. The LLC in both po- and acc-CL were significantly greater in the hCG group compared to GnRH and Control groups (P
ISSN:0378-4320
1873-2232
1873-2232
DOI:10.1016/j.anireprosci.2024.107474