Metal accumulation in female green sea turtles (Chelonia mydas) from Eastern Atlantic affects their egg quality with potential implications for embryonic development

• Published in: The Science of the total environment 2024-06, Vol.931, p.172710-172710, Article 172710
• Main Authors: Morão, Inês F.C., Simões, Tiago, Casado, Roger B., Vieira, Sara, Ferreira-Airaud, Betânia, Caliani, Ilaria, Di Noi, Agata, Casini, Silvia, Fossi, Maria C., Lemos, Marco F.L., Novais, Sara C.
• Format: Article
• Language: English
• Subjects: Animals; Embryonic Development - drug effects; Environmental Monitoring; Erythrocytic nuclear abnormalities; Fatty acids; Female; Genotoxicity; Metals; Ovum - chemistry; Pollution load index; Reproduction; Trace elements; Turtles - embryology; Water Pollutants, Chemical - analysis
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.172710

Sea turtles, with their global distribution and complex life cycle, often accumulate pollutants such as metals and metalloids due to their extended lifespan and feeding habits. However, there are limited studies exploring the impact of metal pollution on the reproductive health of female sea turtles, specifically focusing on the quality of their eggs, which has significant implications for the future generations of these charismatic animals. São Tomé Island, a crucial nesting and feeding habitat for green sea turtles, underscores the urgent need for comprehensive research in this ecologically significant area. This study aimed to investigate whether metals and metalloids in the blood of nesting female green sea turtles induce genotoxic effects in their erythrocytes and affect their egg morphometric characteristics and the composition of related compartments. Additionally, this study aimed to evaluate whether the quality of energetic reserves for embryo development (fatty acids in yolk's polar and neutral lipids) is influenced by the contamination status of their predecessors. Results revealed correlations between Cu and Hg levels and increased “lobed” erythrocytes, while As and Cu negatively influenced shell thickness. In terms of energy reserves, both polar and neutral lipid fractions contained primarily saturated and monounsaturated fatty acids, with prevalent 18:1n-9, 18:0, 16:0, 14:0, and 12:0 fatty acids in yolk samples. The yolk polar fraction was more susceptible to contaminant levels in female sea turtles, showing consistent negative correlations between pollution load index and essential n3 fatty acids, including linolenic, eicosatrienoic, eicosapentaenoic, and docosapentaenoic acids, crucial for embryonic development. These metals accumulation, coupled with the reduced availability of these key fatty acids, may disrupt the eicosanoid and other important pathways, affecting reproductive development. This study reveals a negative correlation between metal contamination in female sea turtles' blood and egg lipid reserves, raising concerns about embryonic development and the species' future generations. [Display omitted] •Cu and Hg levels associated with erythrocytes' abnormalities in nesting females.•Increased Cu and As levels associated with a decrease in eggshell thickness.•Pollution load index links consistently with a decrease of vital FAs in egg yolk.•Increased metal levels associated with a decrease in the quality of egg reserves.•Metals in fe