Eosinophil peroxidase promotes bronchial epithelial cells to secrete asthma-related factors and induces the early stage of airway remodeling

Asthma is a heterogeneous disease characterized by chronic airway inflammation, reversible airflow limitation, and airway remodeling. Eosinophil peroxidase (EPX) is the most abundant secondary granule protein unique to activated eosinophils. In this study, we aimed to illustrate the effect of EPX on...

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Bibliographic Details
Published in:Clinical immunology (Orlando, Fla.) Fla.), 2024-06, Vol.263, p.110228, Article 110228
Main Authors: Xu, Liping, Huang, Xuemei, Chen, Zhangrong, Yang, Meiling, Deng, Jingmin
Format: Article
Language:English
Subjects:
ADAM Proteins
Adult
Airway Remodeling
Asthma
Asthma - immunology
Asthma - metabolism
Asthma - pathology
Asthma - physiopathology
Bronchi - pathology
Cell Line
Cell Movement
Cell Proliferation
Chromones - pharmacology
Cytokines - metabolism
Eosinophil peroxidase
Eosinophil Peroxidase - metabolism
Epithelial Cells - metabolism
Epithelial-Mesenchymal Transition
Female
Humans
Inflammatory
Interleukin-5 - metabolism
Male
Middle Aged
Morpholines - pharmacology
Thymic Stromal Lymphopoietin
Transforming Growth Factor beta1 - metabolism
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Summary:Asthma is a heterogeneous disease characterized by chronic airway inflammation, reversible airflow limitation, and airway remodeling. Eosinophil peroxidase (EPX) is the most abundant secondary granule protein unique to activated eosinophils. In this study, we aimed to illustrate the effect of EPX on the epithelial-mesenchymal transition (EMT) in BEAS-2B cells. Our research found that both EPX and ADAM33 were negatively correlated with FEV1/FVC and FEV1%pred, and positively correlated with IL-5 levels. Asthma patients had relatively higher levels of ADAM33 and EPX compared to the healthy control group. The expression of TSLP, TGF-β1 and ADAM33 in the EPX intervention group was significantly higher. Moreover, EPX could promote the proliferation, migration and EMT of BEAS-2B cells, and the effect of EPX on various factors was significantly improved by the PI3K inhibitor LY294002. The findings from this study could potentially offer a novel therapeutic target for addressing airway remodeling in bronchial asthma, particularly focusing on EMT.
ISSN:1521-6616
1521-7035
1521-7035
DOI:10.1016/j.clim.2024.110228