SARS-CoV-2 live virus culture and sample freeze-thaw stability

•SARS-CoV-2 patient samples covered the range of cycle threshold values.•Split samples were cultured fresh and after storage at 80 °C for 7 or 17-18 days.•Freeze-thaw did not significantly affect SARS-CoV-2 viral culture isolation.•Therefore, frozen samples may be used to assess SARS-CoV-2 infectiou...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2024-07, Vol.109 (3), p.116282-116282, Article 116282
Main Authors: Kanki, Phyllis J., Hamel, Donald J., Riedel, Stefan, Dutta, Sanjucta, Cheng, Annie, Chang, Charlotte A., Arnaout, Ramy, Kirby, James E.
Format: Article
Language:English
Subjects:
COVID-19 - virology
Cryopreservation
Freeze-thaw
Freezing
Humans
Microbial Viability
Sample stability
SARS-CoV-2
Specimen Handling - methods
Virus Cultivation - methods
Virus culture
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•SARS-CoV-2 patient samples covered the range of cycle threshold values.•Split samples were cultured fresh and after storage at 80 °C for 7 or 17-18 days.•Freeze-thaw did not significantly affect SARS-CoV-2 viral culture isolation.•Therefore, frozen samples may be used to assess SARS-CoV-2 infectiousness. The effect of freeze-thaw on SARS-CoV-2 viral viability is not well established. We isolated virus from 31 split clinical samples cultured fresh or after a 7- or 17/18-day freeze. We found that freeze-thaw did not significantly affect viral culture isolation. Therefore, frozen samples may be used to assess SARS-CoV-2 infectiousness.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2024.116282