Low toxicity of magnetite-based modified bionanocomposites with potential application for wastewater treatment: Evaluation in a zebrafish animal model

• Published in: Chemosphere (Oxford) 2024-06, Vol.358, p.142081-142081, Article 142081
• Main Authors: Guillén-Pacheco, Amaimen, Ardila, Yeferzon, Peñaranda, Paula Andrea, Bejarano, Miranda, Rivas, Ricardo, Osma, Johann F., Akle, Veronica
• Format: Article
• Language: English
• Subjects: Bioaccumulation; Laccase; Magnetite-based bionanocomposites; Survival; Zebrafish
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.142081

In recent years, the escalating concerns surrounding environmental pollution and the need for sustainable wastewater treatment solutions have underscored the significance of developing technologies that can efficiently treat wastewater while also reducing negative ecological effects. In this context, our study aims to contribute to the advancement of sustainable technologies for wastewater treatment, by investigating the effects that bare magnetite nanoparticles and those functionalized with the enzyme laccase could have in an aquatic animal, zebrafish, at various life cycle stages. Exposure to magnetite nanoparticles shows some effects on embryo hatching, survival rates, or larval behavior at higher concentrations. For both treatments, the hatching percentages were close to 80% compared to 93% for the control group. At the end of the observations in larvae, survival in all the evaluated groups was higher than 90%. Additionally, we evaluated the accumulation of nanoparticles in various stages of zebrafish. We found that, although there was accumulation during embryonic stages, it did not affect normal development or subsequent hatching. Iron levels in different organs such as gills, muscles, gastrointestinal tract, and brain were also evaluated in adults. Animals treated with a mix of food and nanoparticles at 10 μg/mL (Food group) presented a higher concentration of iron accumulation in muscle, gastrointestinal tract, and gills compared to the untreated control group. Although iron levels increased depending on the dose and exposure method applied, they were not statistically significant from the control groups. Our findings suggest that bionanocomposites evaluated here can be considered safe for removal of contaminants in wastewater without toxic effects or detrimental accumulation fish's health. [Display omitted] •Zebrafish exhibit no significant adverse effects from magnetite throughout their life cycle.•Increasing nanoparticle concentrations may affect toxicological parameters in zebrafish.•The accumulation of bare magnetite nanoparticles minimally affects iron levels.•Magnetite-based bionanocomposites can be considered for wastewater treatment.