Functional Divergence of the Paralog Salmonella Effector Proteins SopD and SopD2 and Their Contributions to Infection

• Published in: International journal of molecular sciences 2024-04, Vol.25 (8), p.4191
• Main Authors: Oke, Mosopefoluwa T, D'Costa, Vanessa M
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Evolution, Molecular; Fever; Gastroenteritis; Genes; Genomes; Genotype & phenotype; Host-Pathogen Interactions - genetics; Humans; Infections; Localization; Pathogenesis; Proteins; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; Salmonella; Salmonella enterica - genetics; Salmonella enterica - metabolism; Salmonella enterica - pathogenicity; Salmonella Infections - metabolism; Salmonella Infections - microbiology; Virulence; Virulence Factors - genetics; Virulence Factors - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25084191

is a leading cause of bacterial food-borne illness in humans and is responsible for millions of cases annually. A critical strategy for the survival of this pathogen is the translocation of bacterial virulence factors termed effectors into host cells, which primarily function via protein-protein interactions with host proteins. The genome encodes several paralogous effectors believed to have arisen from duplication events throughout the course of evolution. These paralogs can share structural similarities and enzymatic activities but have also demonstrated divergence in host cell targets or interaction partners and contributions to the intracellular lifecycle of . The paralog effectors SopD and SopD2 share 63% amino acid sequence similarity and extensive structural homology yet have demonstrated divergence in secretion kinetics, intracellular localization, host targets, and roles in infection. SopD and SopD2 target host Rab GTPases, which represent critical regulators of intracellular trafficking that mediate diverse cellular functions. While SopD and SopD2 both manipulate Rab function, these paralogs display differences in Rab specificity, and the effectors have also evolved multiple mechanisms of action for GTPase manipulation. Here, we highlight this intriguing pair of paralog effectors in the context of host-pathogen interactions and discuss how this research has presented valuable insights into effector evolution.