Actinomycetospora termitidis sp. nov., an insect-derived actinomycete isolated from termite (Odontotermes formosanus)

• Published in: Journal of antibiotics 2024-05, Vol.77 (5), p.299-305
• Main Authors: Supong, Khomsan, Niemhom, Nantawan, Suriyachadkun, Chanwit, Phongsopitanun, Wongsakorn, Tanasupawat, Somboon, Pittayakhajonwut, Pattama
• Format: Article
• Language: English
• Subjects: 14/28; 14/63; 38/22; 38/23; 38/43; 38/77; 38/91; 45/22; 45/61; 631/158/670; 631/326/41/2173; Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinomycetospora; Animals; Antibiotics; Arabinose; Bacteriology; Base Composition; Biomedical and Life Sciences; Bioorganic Chemistry; Cardiolipin; Deoxyribonucleic acid; Diaminopimelic Acid; DNA; DNA, Bacterial - genetics; Galactose; Genetic engineering; Genomes; Genomic analysis; Hybridization; Hydrolysates; Insects; Isoptera - microbiology; Life Sciences; Medicinal Chemistry; Menaquinones; Methods; Microbiology; New species; Nucleotides; Odontotermes formosanus; Organic Chemistry; Peptidoglycans; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phospholipids - chemistry; Phylogenetics; Phylogeny; Physiology; Ribose; RNA, Ribosomal, 16S - genetics; rRNA; Science; Sequence Analysis, DNA; Software; Strain analysis; Substrates; Thailand; Vitamin K 2 - chemistry
• ISSN: 0021-8820; 1881-1469; 1881-1469
• DOI: 10.1038/s41429-024-00712-8

Strain Odt1-22 T , an insect-derived actinomycete was isolated from a termite ( Odontotermes formosanus ) that was collected from Chanthaburi province, Thailand. Strain Odt1-22 T was aerobic, Gram-stain-positive, and produced bud-like spore chain on the substrate hypha. According to chemotaxonomic analysis, strain Odt1-22 T contained meso -diaminopimelic acid in peptidoglycan and the whole-cell hydrolysates contained arabinose, galactose, glucose, and ribose. The major menaquinone was MK-8(H 4 ). The diagnostic phospholipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16 S rRNA gene sequence revealed that strain Odt1-22 T was identified to the genus Actinomycetospora and showed high similarity values with A. chiangmaiensis DSM 45062  T (99.24%), A. soli SF1 T (99.24%) and A. corticicola 014-5  T (98.17%). The genomic size of strain Odt1-22 T was 6.6 Mbp with 73.8% G + C content and 6355 coding sequences (CDSs). The genomic analysis, strain Odt1-22 T and closely related species A. chiangmaiensis DSM 45062  T , A. soli SF1 T and A. corticicola DSM 45772  T displayed the values of average nucleotide identity-blast (ANIb) at 83.7–84.1% and MUMmer (ANIm) at 86.6–87.0%. Moreover, the results of digital DNA-DNA hybridization values between strain Odt1-22 T and related Actinomycetospora species were 45.8−50.5% that lower than the threshold value of commonly used to delineate separated species level. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain Odt1-22 T represented a novel species within the genus Actinomycetospora , for which the name Actinomycetospora termitidis sp. nov. is proposed. The type strain of the species is Odt1-22 T (= TBRC 16192  T  = NBRC 115965  T ).