Potential of melatonin and Trichoderma harzianum inoculation in ameliorating salt toxicity in watermelon: Insights into antioxidant system, leaf ultrastructure, and gene regulation

• Published in: Plant physiology and biochemistry 2024-06, Vol.211, p.108639-108639, Article 108639
• Main Authors: Ghani, Muhammad Imran, Yi, Benlin, Rehmani, Muhammad Saad, Wei, Xi, Siddiqui, Junaid Ali, Fan, Ruidong, Liu, Yanjiang, El-Sheikh, Mohamed A., Chen, Xiaoyulong, Ahmad, Parvaiz
• Format: Article
• Language: English
• Subjects: Antioxidant system; Leaf ultrastructure; Melatonin seed priming; Salt stress; Trichoderma harzianum; Watermelon
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108639

Melatonin (MT) is an extensively studied biomolecule with dual functions, serving as an antioxidant and a signaling molecule. Trichoderma Harzianum (TH) is widely recognized for its effectiveness as a biocontrol agent against many plant pathogens. However, the interplay between seed priming and MT (150 μm) in response to NaCl (100 mM) and its interaction with TH have rarely been investigated. This study aimed to evaluate the potential of MT and TH, alone and in combination, to mitigate salt stress (SS) in watermelon plants. The findings of this study revealed a significant decline in the morphological, physiological, and biochemical indices of watermelon seedlings exposed to SS. However, MT and TH treatments reduced the negative impact of salt stress. The combined application of MT and TH exerted a remarkable positive effect by increasing the growth, photosynthetic and gas exchange parameters, chlorophyll fluorescence indices, and ion balance (decreasing Na+ and enhancing K+). MT and TH effectively alleviated oxidative injury by inhibiting hydrogen peroxide formation in saline and non-saline environments, as established by reduced lipid peroxidation and electrolyte leakage. Moreover, oxidative injury induced by SS on the cells was significantly mitigated by regulation of the antioxidant system, AsA-GSH-related enzymes, the glyoxalase system, augmentation of osmolytes, and activation of several genes involved in the defense system. Additionally, the reduction in oxidative damage was examined by chloroplast integrity via transmission electron microscopy (TEM). Overall, the results of this study provide a promising contribution of MT and TH in safeguarding the watermelon crop from oxidative damage induced by salt stress. •Salt stress (SS) reduced watermelon growth and augmented oxidative stress.•Melatonin (MT) and Trichoderma harzianum (TH) mitigate salt toxicity in watermelon.•MT and TH alleviated oxidative stress by upregulating antioxidant enzymes.•MT and TH enhanced non-enzymatic antioxidants and osmolytes to reduce oxidative stress.•The combined MT and TH was more effective against SS than individual application.