Effects of yak rumen anaerobic fungus Orpinomyces sp. YF3 fermented on in vitro wheat straw fermentation and microbial communities in dairy goat rumen fluid, with and without fungal flora

• Published in: Journal of animal physiology and animal nutrition 2024-09, Vol.108 (5), p.1312-1325
• Main Authors: Du, Xueer, Zhou, Linlin, Li, Yong, Zhang, Fan, Wang, Lamei, Yao, Junhu, Chen, Xinghua, Liu, Shimin, Cao, Yangchun
• Format: Article
• Language: English
• Subjects: Abundance; Acetic acid; Acid production; Ammonia; anaerobic fungi; Anaerobic microorganisms; Anaerobic treatment; Animal Feed - analysis; Animals; Bacteria; Bacteria - classification; Biodegradation; Cell walls; Cellulase; Cellulose; Cellulose propionates; Cycloheximide; Degradation; Factorial design; Feed additives; Female; Fermentation; Fermented milk products; fibre degrading enzyme; Flora; Fungal flora; Fungi; Gastrointestinal Microbiome - drug effects; Goats; Microbial activity; Microbiota; Microorganisms; Nutrients; Orpinomyces; Plant layout; Propionic acid; Relative abundance; Rumen - microbiology; rumen microorganism; Rumen microorganisms; Straw; Technology assessment; Triticum - chemistry; Variance analysis; Volatile fatty acids; Wheat; Wheat straw; yak
• ISSN: 0931-2439; 1439-0396; 1439-0396
• DOI: 10.1111/jpn.13978

Rumen fungi play an essential role in the breakdown of dietary fibrous components, facilitating the provision of nutrients and energy to the host animals. This study investigated the fermentation characteristics and effects on rumen microbiota of yak rumen anaerobic fungus Orpinomyces sp. YF3 in goat rumen fluid, both with and without fungal flora, utilizing anaerobic fermentation bottles. Crushed and air‐dried wheat straw served as the fermentation substrate, and cycloheximide was used to eradicate microorganisms from the rumen fluid of dairy goats. The experiment compromised four treatment groups (2×2 factorial design): control (C); yak fungus group (CF, Orpinomyces sp. YF3); goat fungi eliminated group (CA, antibiotic: 0.25 mg/mL cycloheximide); goat fungi eliminated+yak fungus group (CAF). Each treatment had six replicates. Fermentation characteristics and microbial composition of the fermentation media were analyzed using one‐way analysis of variance and high‐throughput sequencing technology. The findings revealed that in the Orpinomyces sp. YF3 addition group (CF and CAF groups), there were significant increases in ammonia nitrogen concentration by 70%, total volatile fatty acids (VFA) by 53%, as well as acetate, isobutyrate, and valerate concentrations, and the ratio of acetate to propionate (p