The wheat pathogenesis-related protein (TdPR1.2) enhanced tolerance to abiotic and biotic stresses in transgenic Arabidopsis plants

• Published in: Protoplasma 2024-09, Vol.261 (5), p.1035-1049
• Main Authors: Zribi, Ikram, Ghorbel, Mouna, Jrad, Olfa, Masmoudi, Khaled, Brini, Faiçal
• Format: Article
• Language: English
• Subjects: Abiotic stress; Abscisic acid; Alternaria - physiology; Arabidopsis; Arabidopsis - genetics; Arabidopsis - microbiology; Biomedical and Life Sciences; Botrytis; Cell Biology; Cyclopentanes - metabolism; Cyclopentanes - pharmacology; Gene Expression Regulation, Plant - drug effects; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Jasmonic acid; Life Sciences; Original Article; Oxidative Stress; Oxylipins - metabolism; Oxylipins - pharmacology; Pathogenesis; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plants, Genetically Modified - genetics; Proteins; Reverse transcription; Salicylic acid; Sodium chloride; Stress, Physiological - genetics; Superoxide dismutase; Transgenic plants; Triticum - genetics; Triticum - microbiology; Triticum durum; Zoology
• ISSN: 0033-183X; 1615-6102; 1615-6102
• DOI: 10.1007/s00709-024-01955-w

In plants, the pathogenesis-related (PR) proteins have been identified as important regulators of biotic and abiotic stresses. PR proteins branch out into 19 different classes (PR1–PR19). Basically, all PR proteins display a well-established method of action, with the notable exception of PR1, which is a member of a large superfamily of proteins with a common CAP domain. We have previously isolated and characterized the first PR1 from durum wheat, called TdPR-1.2. In the current research work, TdPR1.2 gene was used to highlight its functional activities under various abiotic (sodium chloride (100 mM NaCl) and oxidative stresses (3 mM H 2 O 2 ), hormonal salicylic acid (SA), abscisic acid (ABA) and jasmonic acid (JA), and abiotic stresses ( Botrytis cinerea and Alternaria solani ). Enhancement survival index was detected in Arabidopsis transgenic plants expressing TdPR1.2 gene. Moreover, quantitative real-time reverse transcription PCR (qRT-PCR) analysis demonstrated induction of antioxidant enzymes such as catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). It equally revealed a decrease of malondialdehyde (MDA) as well as hydrogen peroxide (H 2 O 2 ) levels in transgenic Arabidopsis plants compared to control lines, confirming the role of TdPR1.2 in terms of alleviating biotic and abiotic stresses in transgenic Arabidopsis plants. Eventually, RT-qPCR results showed a higher expression of biotic stress-related genes (PR1 and PDF1.2) in addition to a downregulation of the wound-related gene (LOX3 and VSP2) in transgenic lines treated with jasmonic acid (JA). Notably, these findings provide evidence for the outstanding functions of PR1.2 from durum wheat which can be further invested to boost tolerance in crop plants to abiotic and biotic stresses.