The efficacy of chemical inducers and fungicides in controlling tomato root rot disease caused by Rhizoctoniasolani

• Published in: Plant physiology and biochemistry 2024-05, Vol.210, p.108669-108669, Article 108669
• Main Authors: Mogazy, Asmaa M., Abdallah, Wafaa E., Mohamed, Heba I., Omran, Ahmed A.A.
• Format: Article
• Language: English
• Subjects: Diseases severity; Flavonoid fraction; HPLC; Isoflavone fraction; Isozymes; Pathogenesis related genes; Phenolic fractions; Protein electrophoresis
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108669

Chitosan is an environmentally friendly natural substance that is used in crop disease management as an alternative to chemical pesticides. A significant issue restricting output in Egypt is root rot, which is a disease, caused by Rhizoctonia solani. Therefore, a greenhouse experiment was conducted to assess the effects of R. solani on 60-day-old tomato plants under fungal infection and to determine the antifungal activity of chitosan and Rizolax T fungicide against the pathogenic fungus. The findings demonstrated that 4 g/L of chitosan seed application completely obstructed the radial mycelial growth of R. solani and decreased the disease severity. Pathogenic infection significantly decreased morphological characteristics and total chlorophyll but significantly increased carotenoid, total thiol, non-protein thiol, protein thiol, antioxidant enzymes, oxidative stress, total phenolic, total flavonoid, and isoflavone compared to healthy plants. Tomato plants treated with chitosan exhibited lower rates of oxidative stress, but higher levels of all previously mentioned parameters compared to untreated infected plants. The number and molecular mass of protein banding patterns varied in all treated tomato plants as compared to the healthy control. There are 42 bands in the treatments, and their polymorphism rate is 69.55%. Moreover, the number and density of α- and β-esterase, and peroxidase isozymes in treated tomato plants exhibited varied responses. Moreover, in treated and control plants, chitosan treatment raised the expression levels of phenylalanine ammonia-lyase, pathogenesis-related protein-1, β-1,3-glucanases and chitinase. In conclusions, chitosan reduces R. solani infection by controlling the biochemical and molecular mechanisms in tomato plants during infection. •Root rot considered the most dangerous tomato diseases.•Fungicide use is one of the oldest methods for disease control.•Chitosan considered as a synthetic plant defense elicitor in the last two decades.•Chitosan inhibits mycelial growth and development of R. solani.•Chitosan regulates the expression of many genes.