Molecular characterization and expression profile of the ALDH1A1 gene and its functions in yak luteal cells

• Published in: Theriogenology 2024-07, Vol.223, p.98-107
• Main Authors: Fei, Xixi, Zhu, Yanjin, Pan, Bangting, Cheng, Yuying, Yang, Qinhui, Xie, Yumian, Xiong, Yan, Fu, Wei, Xiong, Xianrong, Li, Jian
• Format: Article
• Language: English
• Subjects: Aldehyde Dehydrogenase - genetics; Aldehyde Dehydrogenase - metabolism; Aldehyde Dehydrogenase 1 Family - genetics; Aldehyde Dehydrogenase 1 Family - metabolism; ALDH1A1; Animals; Apoptosis; Cattle - genetics; Cell Proliferation; Female; Gene expression; Gene Expression Regulation - physiology; Luteal cells; Luteal Cells - metabolism; Progesterone - metabolism; Retinal Dehydrogenase - genetics; Retinal Dehydrogenase - metabolism; Steroidogenesis; Yak
• ISSN: 0093-691X; 1879-3231; 1879-3231
• DOI: 10.1016/j.theriogenology.2024.04.020

The ALDH1A1 gene encodes a cytoplasmic member of the aldehyde dehydrogenase 1 family, which plays an important role in regulating animal reproductive performance, including estrus cycle and embryonic development. The aim of this study was to characterize ALDH1A1 activity in ovaries of 3–5 year-old yaks and to determine its effects on cell proliferation, apoptosis, and progesterone secretion in luteal cells (LCs). The coding sequence (CDS) of the ALDH1A1 gene was cloned by reverse transcription-PCR and immunohistochemical analysis was used to confirm localization of the ALDH1A1 protein in the ovary. To assess the activity of ALDH1A1 in regulating progesterone secretion, si-ALDH1A1 was transfected into LCs in vitro and progesterone levels in LC supernatants were measured by ELISA. The interference efficiency was assessed by real-time quantitative PCR (RT-qPCR) and immunofluorescence staining, and cell proliferation and apoptosis were evaluated by EdU and TUNEL staining, respectively. The cloned ALDH1A1 sequence contained 1462 bp, encoding 487 amino acids. Immunohistochemical analysis showed that ALDH1A1 protein expression, which was significantly higher in LCs, was mainly found in antral follicles and the corpus luteum (CL). The expression of ALDH1A1 mRNA in LCs was effectively inhibited by si-ALDH1A1transfection, and progesterone secretion was markedly decreased along with the significant down-regulation of progesterone pathway-related genes, STAR, CYP11A1, CYP19A1, CYP17A1, 3β-HSD, and HSD17B1. Knockdown of ALDH1A1 mRNA expression decreased cell proliferation and increased apoptosis in LCs. The mRNA expression of the proliferation-related genes, PCNA, CCND1, CCNB1 and CDC25A, was significantly down-regulated, while expression of the apoptosis-promoting CASP3 gene was significantly increased. In summary, we characterized the yak ALDH1A1 gene and revealed that ALDH1A1 knockdown promoted apoptosis, repressed cell proliferation, and decreased progesterone secretion by yak LCs, potentially by regulating the mRNA expression of genes related to proliferation, apoptosis, and progesterone synthesis and secretion. •The expression levels of ALDH1A1 in luteal cells(LCs) are significantly higher than that in both granulosa cells (GCs) and theca cells (TCs);•Knockdown of ALDH1A1 expression induced a decrease in cell proliferation and an increase in the apoptosis rate of LCs;•ALDH1A1 regulated the biological function of yak LCs by modulating the expression of progesteron