Heat Treatment and Homogenization Influence the Gastric Digestion of Bovine Milk Protein in Growing Pigs as an Adult Human Model

• Published in: The Journal of nutrition 2024-07, Vol.154 (7), p.2097-2107
• Main Authors: Ahlborn, Natalie G, Montoya, Carlos A, Roy, Nicole C, Ye, Aiqian, Samuelsson, Linda M, Wieliczko, Robert J, McNabb, Warren C
• Format: Article
• Language: English
• Subjects: Amino acids; Amino groups; bovine milk; Cattle; Cow's milk; digested protein gastric emptying; Digestion; Digestive system; Electrophoresis; Emptying; gastric amino acid emptying; Gastric emptying; Heat treatment; Heat treatments; High temperature; Homogenization; Hydrolysis; Intestine; Milk; Nutrition; Pasteurization; Polyacrylamide; Protein folding; protein hydrolysis; Protein structure; Proteins; Small intestine; Sodium dodecyl sulfate; Sodium lauryl sulfate; Swine; Ultrahigh temperature
• ISSN: 0022-3166; 1541-6100; 1541-6100
• DOI: 10.1016/j.tjnut.2024.04.035

Bovine milk processing influences the structure of the curd formed during gastric digestion, which may alter gastric protein hydrolysis and impact amino acid (AA) release into the small intestine. This study aimed to determine the influence of heat treatment and homogenization on the gastric protein digestion and AA emptying of bovine milk. Nine-wk-old pigs (n = 144) consumed either raw, pasteurized nonhomogenized (PNH), pasteurized homogenized (PH), or ultra-high-temperature homogenized (UHT) bovine milk for 10 d. On day 11, fasted pigs received the milk treatment (500 mL) before gastric contents were collected at 0, 20, 60, 120, 180, and 300 min postprandially. The apparent degree of gastric protein hydrolysis (based on the release of free amino groups), apparent gastric disappearance of individual proteins [based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel band intensity], and the gastric emptying of digested protein and AA were determined. During the first 60 min, the rate of apparent gastric protein hydrolysis was fastest in pigs fed UHT milk (0.29%/min compared with on average 0.07%/min in pigs fed raw, PNH, and PH milk). Differences in the apparent degree of gastric protein hydrolysis and emptying were reflected in the rate of digested protein entering the small intestine. The AA gastric emptying half-time was generally shorter in pigs fed PH and UHT milk than in pigs fed raw and PNH milk. For example, the gastric release of total essential AA was >2-fold faster (P < 0.01) in pigs fed PH or UHT milk than that in pigs fed raw or PNH milk (i.e., homogenized compared with nonhomogenized milk). Heat treatment and homogenization increased the apparent gastric degree of protein hydrolysis and the release of digested protein into the small intestine. However, the rate of AA entering the small intestine was mainly increased by homogenization.