Rapid and reliable ratiometric fluorescence detection of isoquercitrin based on a high-nuclearity Zn(II)-Nd(III) nanomolecular sensor

• Published in: Talanta (Oxford) 2024-08, Vol.275, p.126170-126170, Article 126170
• Main Authors: Zhao, Jinni, Yang, Xiaoping, Leng, Xilong, Wang, Chengri, Schipper, Desmond
• Format: Article
• Language: English
• Subjects: High-nuclearity Zn–Nd nanomolecular sensor; Isoquercitrin; Lanthanide luminescence; Ratiometric fluorescence detection; Schiff base ligand
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2024.126170

Rapid and quantitative detection of isoquercitrin (Isq) has been attracting much attention due to its outstanding pharmacological and physiological activities. Herein, an interesting 48-metal Zn(II)-Nd(III) nanocluster (1, molecular sizes 1.3 × 2.8 × 3.1 nm) with salen-type Schiff base ligand was constructed as molecular sensor for the luminescence detection of Isq. 1 exhibits visible ligand-centered emission and NIR luminescence of Nd(III), and shows ratiometric fluorescence response to Isq with high sensitivity even in the presence of other interferences. The fluorescence sensing behavior can be expressed by a second-order equation I1060nm/I480nm = A*[Isq]2 + B*[Isq] + C, which is used to quantitatively analyze the Isq concentrations in DMF and FCS. The LODs to Isq for the ligand-centered and lanthanide emissions of 1 in DMF are 0.21 μM and 0.11 nM, respectively. The quenching of the ligand-centered emission of 1 caused by Isq is attributed to the competitive absorption of light energy and “inner effect”, while, the luminescence enhancement is due to the “antenna effect”. [Display omitted] •Isoquercitrin (Isq) is detected firstly by a NIR luminescent high-nuclearity d-4f nanomolecular sensor.•The ratiometric fluorescence detection of Isq is fast and reliable.•The ratiometric fluorescence detection of Isq is highly sensitive and selective.