Role of N6-methyladenosine-related lncRnas in pseudoexfoliation glaucoma

To explore the role of lncRNA m 6 A methylation modification in aqueous humour (AH) of patients with pseudoexfoliation glaucoma (PXG). Patients with open-angle PXG under surgery from June 2021 to December 2021 were selected. Age- and gender-matched patients with age-related cataract (ARC) were chose...

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Published in:Epigenetics 2024-12, Vol.19 (1), p.2348840-2348840
Main Authors: Guan, Jieying, Chen, Xiaohong, Li, Zhidong, Deng, Shuifeng, Wumaier, Aizezi, Ma, Yuncheng, Xie, Lingling, Huang, Shengsong, Zhu, Yingting, Zhuo, Yehong
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - genetics
Adenosine - metabolism
Aged
Aqueous Humor - metabolism
aqueous humour
DNA Methylation
Exfoliation Syndrome - genetics
Exfoliation Syndrome - metabolism
Female
Gene Regulatory Networks
Glaucoma, Open-Angle - genetics
Glaucoma, Open-Angle - metabolism
Humans
lncRNA
Male
methylation
Middle Aged
Pseudoexfoliation glaucoma
rho-Associated Kinases - genetics
rho-Associated Kinases - metabolism
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
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Summary:To explore the role of lncRNA m 6 A methylation modification in aqueous humour (AH) of patients with pseudoexfoliation glaucoma (PXG). Patients with open-angle PXG under surgery from June 2021 to December 2021 were selected. Age- and gender-matched patients with age-related cataract (ARC) were chosen as control. Patients underwent detailed ophthalmic examinations. 0.05-0.1 ml AH were extracted during surgery for MeRIP-Seq and RNA-Seq. Joint analysis was used to screen lncRNAs with differential m 6 A methylation modification and expression. Online software tools were used to draw lncRNA-miRNA-mRNA network (ceRNA). Expression of lncRNAs and mRNAs was confirmed using quantitative real-time PCR. A total of 4151 lncRNAs and 4386 associated m 6 A methylation modified peaks were identified in the PXG group. Similarly, 2490 lncRNAs and 2595 associated m 6 A methylation modified peaks were detected in the control. Compared to the ARC group, the PXG group had 234 hypermethylated and 402 hypomethylated m 6 A peaks, with statistically significant differences (| Fold Change (FC) |≥2, p 
ISSN:1559-2294
1559-2308
DOI:10.1080/15592294.2024.2348840