3D printing-based lateral flow visual assay utilizing the dual-excitation property of nitrogen-doped carbon dots for the ratiometric determination and chemometric discrimination of flavonoids

A ratiometric fluorescence sensor has been established based on dual-excitation carbon dots (D-CDs) for the detection of flavonoids (morin is chosen as the typical detecting model for flavonoids). D-CDs were prepared using microwave radiation with o-phenylenediamine and melamine and exhibit controll...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2024-06, Vol.191 (6), p.310-310, Article 310
Main Authors: Ma, Danhua, Zhang, Ting, Xing, Haoming, Wang, Linfan, Chen, Da, Wang, Liang
Format: Article
Language:English
Subjects:
Analytical Chemistry
Carbon - chemistry
Carbon dots
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Controllability
Excitation
Flavones
Flavonoids
Flavonoids - analysis
Flavonoids - chemistry
Fluorescent Dyes - chemistry
Humans
Limit of Detection
Melamine
Microengineering
Microwaves
Nanochemistry
Nanotechnology
Nitrogen - chemistry
Original Paper
Phenylenediamine
Printing, Three-Dimensional
Quantum Dots - chemistry
Reference signals
Spectrometry, Fluorescence - methods
Three dimensional flow
Three dimensional printing
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Summary:A ratiometric fluorescence sensor has been established based on dual-excitation carbon dots (D-CDs) for the detection of flavonoids (morin is chosen as the typical detecting model for flavonoids). D-CDs were prepared using microwave radiation with o-phenylenediamine and melamine and exhibit controllable dual-excitation behavior through the regulation of their concentration. Remarkably, the short-wavelength excitation of D-CDs can be quenched by morin owing to the inner filter effect, while the long-wavelength excitation remains insensitive, serving as the reference signal. This contributes to the successful design of an excitation-based ratiometric sensor. Based on the distinct and differentiated variation of excitation intensity, morin can be determined from 0.156 to 110 µM with a low detection limit of 0.156 µM. In addition, an intelligent and visually lateral flow sensing device is developed for the determination  of morin content in real samples with satisfying recoveries, which indicates the potential application for human health monitoring. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-024-06383-4