Evaluation of quercetin in alleviating the negative effects of high soybean meal diet on spotted sea bass Lateolabrax maculatus

• Published in: Fish & shellfish immunology 2024-07, Vol.150, p.109607-109607, Article 109607
• Main Authors: Liu, Longhui, Zhao, Yanbo, Huang, Zhangfan, Long, Zhongying, Qin, Huihui, Lin, Hao, Zhou, Sishun, Kong, Lumin, Ma, Jianrong, Lin, Yi, Li, Zhongbao
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animals; Bass - immunology; Diet - veterinary; Dietary Supplements - analysis; Dose-Response Relationship, Drug; Gastrointestinal Microbiome - drug effects; Glycine max - chemistry; High soybean meal diet; Intestinal microbiota; Intestinal Mucosa - drug effects; Metabolomics; Quercetin; Quercetin - administration & dosage; Quercetin - pharmacology; Random Allocation; Spotted sea bass
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2024.109607

The aim of this study was to investigate the effects of quercetin (QUE) on alleviating the negative effects of high soybean meal diet for spotted sea bass Lateolabrax maculatus. A healthy control group fed a 44% fishmeal diet was used, while the induction control group replaced 50% fishmeal with soybean meal. Subsequently, QUE was added at concentrations of 0.25, 0.50, 0.75, and 1.00 g/kg in the experimental groups. A total of 540 tailed spotted sea bass were randomly divided into 6 groups and fed the corresponding diet for 56 days. The results showed that 40% soybean meal significantly decreased the growth performance and immunity, increased the intestinal mucosal permeability, and caused damage to the intestinal tissue morphology; moreover, there were alterations observed in the composition of the intestinal microbiota, accompanied by detectable levels of saponins in the metabolites. However, the addition of QUE did not yield significant changes in growth performance; instead, it notably reduced the permeability of the intestinal mucosa, improved the body's immunity and the structural integrity of the intestinal tissue, increased the proportion of Proteobacteria, and enhanced the richness and diversity of intestinal microorganisms to a certain extent. In addition, QUE up-regulate the metabolism of amino acids and their derivatives and energy-related metabolites such as uridine and guanosine; furthermore, it appears to regulate transporters through the ABC transporters pathway to promote the absorption and utilization of QUE by enterocytes. •Try to study whether quercetin can promote soybean meal bioavailability.•Combined with high-throughput 16s rRNA and metabolomics in-depth analysis.•The potential of quercetin in alleviating intestinal damage was revealed.