Analysis of Methylesterase Gene Family in Fragaria vesca Unveils Novel Insights into the Role of FvMES2 in Methyl Salicylate-Mediated Resistance against Strawberry Gray Mold

• Published in: Journal of agricultural and food chemistry 2024-05, Vol.72 (20), p.11392-11404
• Main Authors: Jia, Ruimin, Xing, Keyan, Tian, Lin, Dong, Xiaomin, Yu, Ligang, Shen, Xihui, Wang, Yang
• Format: Article
• Language: English
• Subjects: Agricultural and Environmental Chemistry; Botrytis; Disease Resistance - genetics; Fragaria - enzymology; Fragaria - genetics; Fragaria - microbiology; Fruit - enzymology; Fruit - genetics; Fruit - microbiology; Gene Expression Regulation, Plant; Molecular Docking Simulation; Multigene Family; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Salicylates - metabolism
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c01447

Methylesterases (MESs) hydrolyze carboxylic ester and are important for plant metabolism and defense. However, the understanding of MES’ role in strawberries against pathogens remains limited. This study identified 15 FvMESs with a conserved catalytic triad from the Fragaria vesca genome. Spatiotemporal expression data demonstrated the upregulated expression of FvMESs in roots and developing fruits, suggesting growth involvement. The FvMES promoter regions harbored numerous stress-related cis-acting elements and transcription factors associated with plant defense mechanisms. Moreover, FvMES2 exhibited a significant response to Botrytis cinerea stress and showed a remarkable correlation with the salicylic acid (SA) signaling pathway. Molecular docking showed an efficient binding potential between FvMES2 and methyl salicylate (MeSA). The role of FvMES2 in MeSA demethylation to produce SA was further confirmed through in vitro and in vivo assays. After MeSA was applied, the transient overexpression of FvMES2 in strawberries enhanced their resistance to B. cinerea compared to wild-type plants.