Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging

Purpose This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy. Methods Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U c...

Full description

Saved in:
Bibliographic Details
Published in:Magnetic resonance in medicine 2024-10, Vol.92 (4), p.1658-1669
Main Authors: Guo, Tan, Song, Yan, Tong, Jinlian, Jiao, Sheng, Shen, Cheng, Wang, Hong, Cui, Ju, Dai, Dapeng, Ma, Jie, Chen, Min
Format: Article
Language:English
Subjects:
Aged
Collagen
Collagen - metabolism
collagen degradation
Collagenase
Collagenases - metabolism
Correlation analysis
Degradation
Digestion
Female
Humans
Image Processing, Computer-Assisted - methods
Imaging
In vitro methods and tests
Macromolecules
Magnetic Resonance Imaging - methods
Magnetization
magnetization transfer
Male
Middle Aged
Parameters
Regression analysis
Regression models
Relaxation time
Rotator cuff
Rotator Cuff - diagnostic imaging
Rotator Cuff - metabolism
rotator cuff tendinopathy
Tendinopathy - diagnostic imaging
Tendinopathy - metabolism
Tendons
Tendons - diagnostic imaging
Tendons - metabolism
UTE
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy. Methods Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE‐MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0w) and from water to the macromolecular pool (Rm0m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0w, Rm0m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE‐MT parameters to predict collagen degradation. Results MMF, T2m, R1m, Rm0m, and MTR decreased after digestion. MMF (r = −0.842, p 
ISSN:0740-3194
1522-2594
1522-2594
DOI:10.1002/mrm.30144