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Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging
Purpose This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy. Methods Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U c...
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| Published in: | Magnetic resonance in medicine 2024-10, Vol.92 (4), p.1658-1669 |
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| creator | Guo, Tan Song, Yan Tong, Jinlian Jiao, Sheng Shen, Cheng Wang, Hong Cui, Ju Dai, Dapeng Ma, Jie Chen, Min |
| description | Purpose
This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
Methods
Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE‐MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0w) and from water to the macromolecular pool (Rm0m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0w, Rm0m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE‐MT parameters to predict collagen degradation.
Results
MMF, T2m, R1m, Rm0m, and MTR decreased after digestion. MMF (r = −0.842, p |
| doi_str_mv | 10.1002/mrm.30144 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3053972126</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3083077747</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3134-e47f84b01b97958d10c33422624406f797cf35026ea86ff08f14ca76b65b411e3</originalsourceid><addsrcrecordid>eNp10U1u1DAYBmALUdGhsOACyBKbdpHWf4mTJRoNFKmjSmi6jpzk84yr2B5sp9Ww4ggcgbNxkpqmZYHEyrL86LU_vwi9o-ScEsIubLDnnFAhXqAFLRkrWNmIl2hBpCAFp404Rq9jvCWENI0Ur9AxryUraSMX6NfSj6PagsMDbIMaVDLeYRUjxGjBJXxv0g4rh43DdyYFj4NPKvmA-0lrnMANxvm9SrsDtn6AEU_RuC2205jMXgVlIQXT47wNKu58SL9__NyssFVbB8l8n-_LZy5qCPj0ZrPKYL05wyaTnPQGHWk1Rnj7tJ6gm0-rzfKyuLr-_GX58aroOeWiACF1LTpCu0Y2ZT1Q0nMuGKuYEKTSspG95iVhFai60prUmopeyaqryk5QCvwEnc65--C_TRBTa03sIX-OAz_FlpOSN5JRVmX64R9666fg8uuyqjmRUgqZ1dms-uBjDKDbfcgzhUNLSfuntjbX1j7Wlu37p8SpszD8lc89ZXAxg3szwuH_Se3663qOfABx-aWF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3083077747</pqid></control><display><type>article</type><title>Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Guo, Tan ; Song, Yan ; Tong, Jinlian ; Jiao, Sheng ; Shen, Cheng ; Wang, Hong ; Cui, Ju ; Dai, Dapeng ; Ma, Jie ; Chen, Min</creator><creatorcontrib>Guo, Tan ; Song, Yan ; Tong, Jinlian ; Jiao, Sheng ; Shen, Cheng ; Wang, Hong ; Cui, Ju ; Dai, Dapeng ; Ma, Jie ; Chen, Min</creatorcontrib><description>Purpose
This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
Methods
Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE‐MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0w) and from water to the macromolecular pool (Rm0m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0w, Rm0m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE‐MT parameters to predict collagen degradation.
Results
MMF, T2m, R1m, Rm0m, and MTR decreased after digestion. MMF (r = −0.842, p < 0.001), MTR (r = −0.78, p < 0.001), and Rm0m (r = −0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
Conclusion
This study highlighted the potential of UTE‐MT parameters for evaluation of supraspinatus tendinopathy.</description><identifier>ISSN: 0740-3194</identifier><identifier>ISSN: 1522-2594</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/mrm.30144</identifier><identifier>PMID: 38725197</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Aged ; Collagen ; Collagen - metabolism ; collagen degradation ; Collagenase ; Collagenases - metabolism ; Correlation analysis ; Degradation ; Digestion ; Female ; Humans ; Image Processing, Computer-Assisted - methods ; Imaging ; In vitro methods and tests ; Macromolecules ; Magnetic Resonance Imaging - methods ; Magnetization ; magnetization transfer ; Male ; Middle Aged ; Parameters ; Regression analysis ; Regression models ; Relaxation time ; Rotator cuff ; Rotator Cuff - diagnostic imaging ; Rotator Cuff - metabolism ; rotator cuff tendinopathy ; Tendinopathy - diagnostic imaging ; Tendinopathy - metabolism ; Tendons ; Tendons - diagnostic imaging ; Tendons - metabolism ; UTE</subject><ispartof>Magnetic resonance in medicine, 2024-10, Vol.92 (4), p.1658-1669</ispartof><rights>2024 International Society for Magnetic Resonance in Medicine</rights><rights>2024 International Society for Magnetic Resonance in Medicine.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3134-e47f84b01b97958d10c33422624406f797cf35026ea86ff08f14ca76b65b411e3</cites><orcidid>0000-0003-1149-5849</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38725197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Tan</creatorcontrib><creatorcontrib>Song, Yan</creatorcontrib><creatorcontrib>Tong, Jinlian</creatorcontrib><creatorcontrib>Jiao, Sheng</creatorcontrib><creatorcontrib>Shen, Cheng</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>Cui, Ju</creatorcontrib><creatorcontrib>Dai, Dapeng</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><title>Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging</title><title>Magnetic resonance in medicine</title><addtitle>Magn Reson Med</addtitle><description>Purpose
This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
Methods
Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE‐MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0w) and from water to the macromolecular pool (Rm0m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0w, Rm0m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE‐MT parameters to predict collagen degradation.
Results
MMF, T2m, R1m, Rm0m, and MTR decreased after digestion. MMF (r = −0.842, p < 0.001), MTR (r = −0.78, p < 0.001), and Rm0m (r = −0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
Conclusion
This study highlighted the potential of UTE‐MT parameters for evaluation of supraspinatus tendinopathy.</description><subject>Aged</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>collagen degradation</subject><subject>Collagenase</subject><subject>Collagenases - metabolism</subject><subject>Correlation analysis</subject><subject>Degradation</subject><subject>Digestion</subject><subject>Female</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Imaging</subject><subject>In vitro methods and tests</subject><subject>Macromolecules</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Magnetization</subject><subject>magnetization transfer</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Parameters</subject><subject>Regression analysis</subject><subject>Regression models</subject><subject>Relaxation time</subject><subject>Rotator cuff</subject><subject>Rotator Cuff - diagnostic imaging</subject><subject>Rotator Cuff - metabolism</subject><subject>rotator cuff tendinopathy</subject><subject>Tendinopathy - diagnostic imaging</subject><subject>Tendinopathy - metabolism</subject><subject>Tendons</subject><subject>Tendons - diagnostic imaging</subject><subject>Tendons - metabolism</subject><subject>UTE</subject><issn>0740-3194</issn><issn>1522-2594</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp10U1u1DAYBmALUdGhsOACyBKbdpHWf4mTJRoNFKmjSmi6jpzk84yr2B5sp9Ww4ggcgbNxkpqmZYHEyrL86LU_vwi9o-ScEsIubLDnnFAhXqAFLRkrWNmIl2hBpCAFp404Rq9jvCWENI0Ur9AxryUraSMX6NfSj6PagsMDbIMaVDLeYRUjxGjBJXxv0g4rh43DdyYFj4NPKvmA-0lrnMANxvm9SrsDtn6AEU_RuC2205jMXgVlIQXT47wNKu58SL9__NyssFVbB8l8n-_LZy5qCPj0ZrPKYL05wyaTnPQGHWk1Rnj7tJ6gm0-rzfKyuLr-_GX58aroOeWiACF1LTpCu0Y2ZT1Q0nMuGKuYEKTSspG95iVhFai60prUmopeyaqryk5QCvwEnc65--C_TRBTa03sIX-OAz_FlpOSN5JRVmX64R9666fg8uuyqjmRUgqZ1dms-uBjDKDbfcgzhUNLSfuntjbX1j7Wlu37p8SpszD8lc89ZXAxg3szwuH_Se3663qOfABx-aWF</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Guo, Tan</creator><creator>Song, Yan</creator><creator>Tong, Jinlian</creator><creator>Jiao, Sheng</creator><creator>Shen, Cheng</creator><creator>Wang, Hong</creator><creator>Cui, Ju</creator><creator>Dai, Dapeng</creator><creator>Ma, Jie</creator><creator>Chen, Min</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1149-5849</orcidid></search><sort><creationdate>202410</creationdate><title>Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging</title><author>Guo, Tan ; Song, Yan ; Tong, Jinlian ; Jiao, Sheng ; Shen, Cheng ; Wang, Hong ; Cui, Ju ; Dai, Dapeng ; Ma, Jie ; Chen, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3134-e47f84b01b97958d10c33422624406f797cf35026ea86ff08f14ca76b65b411e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aged</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>collagen degradation</topic><topic>Collagenase</topic><topic>Collagenases - metabolism</topic><topic>Correlation analysis</topic><topic>Degradation</topic><topic>Digestion</topic><topic>Female</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Imaging</topic><topic>In vitro methods and tests</topic><topic>Macromolecules</topic><topic>Magnetic Resonance Imaging - methods</topic><topic>Magnetization</topic><topic>magnetization transfer</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Parameters</topic><topic>Regression analysis</topic><topic>Regression models</topic><topic>Relaxation time</topic><topic>Rotator cuff</topic><topic>Rotator Cuff - diagnostic imaging</topic><topic>Rotator Cuff - metabolism</topic><topic>rotator cuff tendinopathy</topic><topic>Tendinopathy - diagnostic imaging</topic><topic>Tendinopathy - metabolism</topic><topic>Tendons</topic><topic>Tendons - diagnostic imaging</topic><topic>Tendons - metabolism</topic><topic>UTE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Tan</creatorcontrib><creatorcontrib>Song, Yan</creatorcontrib><creatorcontrib>Tong, Jinlian</creatorcontrib><creatorcontrib>Jiao, Sheng</creatorcontrib><creatorcontrib>Shen, Cheng</creatorcontrib><creatorcontrib>Wang, Hong</creatorcontrib><creatorcontrib>Cui, Ju</creatorcontrib><creatorcontrib>Dai, Dapeng</creatorcontrib><creatorcontrib>Ma, Jie</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Tan</au><au>Song, Yan</au><au>Tong, Jinlian</au><au>Jiao, Sheng</au><au>Shen, Cheng</au><au>Wang, Hong</au><au>Cui, Ju</au><au>Dai, Dapeng</au><au>Ma, Jie</au><au>Chen, Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn Reson Med</addtitle><date>2024-10</date><risdate>2024</risdate><volume>92</volume><issue>4</issue><spage>1658</spage><epage>1669</epage><pages>1658-1669</pages><issn>0740-3194</issn><issn>1522-2594</issn><eissn>1522-2594</eissn><abstract>Purpose
This study aims to assess ultrashort‐TE magnetization transfer (UTE‐MT) imaging of collagen degradation using an in vitro model of rotator cuff tendinopathy.
Methods
Thirty‐six supraspinatus tendon specimens were divided into three groups and treated with 600 U collagenase (Group 1), 150 U collagenase (Group 2), and phosphate buffer saline (Group 3). UTE‐MT imaging was performed to assess changes in macromolecular fraction (MMF), macromolecule transverse relaxation time (T2m), water longitudinal relaxation rate constant (R1m), the magnetization exchange rate from the macromolecular to water pool (Rm0w) and from water to the macromolecular pool (Rm0m), and magnetization transfer ratio (MTR) at baseline and following digestion and their differences between groups. Biochemical and histological studies were conducted to determine the extent of collagen degradation. Correlation analyses were performed with MMF, T2m, R1m, Rm0w, Rm0m, and MTR, respectively. Univariate and multivariate linear regression analyses were performed to evaluate combinations of UTE‐MT parameters to predict collagen degradation.
Results
MMF, T2m, R1m, Rm0m, and MTR decreased after digestion. MMF (r = −0.842, p < 0.001), MTR (r = −0.78, p < 0.001), and Rm0m (r = −0.662, p < 0.001) were strongly negatively correlated with collagen degradation. The linear regression model of differences in MMF and Rm0m before and after digestion explained 68.9% of collagen degradation variation in the tendon. The model of postdigestion in MMF and T2m and the model of MTR explained 54.2% and 52.3% of collagen degradation variation, respectively.
Conclusion
This study highlighted the potential of UTE‐MT parameters for evaluation of supraspinatus tendinopathy.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38725197</pmid><doi>10.1002/mrm.30144</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1149-5849</orcidid></addata></record> |
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| subjects | Aged Collagen Collagen - metabolism collagen degradation Collagenase Collagenases - metabolism Correlation analysis Degradation Digestion Female Humans Image Processing, Computer-Assisted - methods Imaging In vitro methods and tests Macromolecules Magnetic Resonance Imaging - methods Magnetization magnetization transfer Male Middle Aged Parameters Regression analysis Regression models Relaxation time Rotator cuff Rotator Cuff - diagnostic imaging Rotator Cuff - metabolism rotator cuff tendinopathy Tendinopathy - diagnostic imaging Tendinopathy - metabolism Tendons Tendons - diagnostic imaging Tendons - metabolism UTE |
| title | Collagen degradation assessment with an in vitro rotator cuff tendinopathy model using multiparametric ultrashort‐TE magnetization transfer (UTE‐MT) imaging |
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