Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species

Neocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogen...

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Bibliographic Details
Published in:Journal of applied microbiology 2024-05, Vol.135 (5)
Main Authors: Klomchit, Anthikan, Calabon, Mark S, Worabandit, Sompradtana, Weaver, Jack A, Karima, Elfina M, Alberti, Fabrizio, Greco, Claudio, Mahanil, Siraprapa
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Ascomycota - genetics
Avicennia - microbiology
Biological Control Agents
Colletotrichum - genetics
DNA, Fungal - genetics
Genome, Fungal
Phylogeny
Plant Diseases - microbiology
Plant Diseases - prevention & control
Thailand
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Summary:Neocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis. Detailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17-0253, MFLUCC 17-0257, and MFLUCC 17-0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17-0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17-0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17-0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters. Two fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17-0253 were obtained.
ISSN:1365-2672
1365-2672
DOI:10.1093/jambio/lxae114