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An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing

Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the fre...

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Published in:International journal of biological macromolecules 2024-06, Vol.269 (Pt 2), p.131859-131859, Article 131859
Main Authors: Rezaei, Elham Salar, Poursamar, Seyed Ali, Naeimi, Mitra, Taheri, Mohammad Mahdi, Rafienia, Mohammad
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container_issue Pt 2
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container_title International journal of biological macromolecules
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creator Rezaei, Elham Salar
Poursamar, Seyed Ali
Naeimi, Mitra
Taheri, Mohammad Mahdi
Rafienia, Mohammad
description Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the frequency of dressing changes. Moreover, the outer layer is a mechanical support for the wound, preventing undue tension in the affected area. A 3D-printed polycaprolactone (PCL) membrane was utilized as the outer layer to fabricate DS wound dressing. Simultaneously, a polyvinyl alcohol/chitosan/sildenafil citrate (PVA/CS/SC) scaffold was electrospun onto the PCL membrane to facilitate cellular adhesion and proliferation. Scanning electron microscopy (SEM) analysis of the PCL filaments revealed a consistent cross-sectional surface and structure, with an average diameter of 562.72 ± 29.15 μm. SEM results also demonstrated uniform morphology and beadless structure for the PVA/CS/SC scaffold, with an average fiber diameter of 366.77 ± 1.81 nm for PVA/CS. The addition of SC led to an increase in fiber diameter while resulting in a reduction in tensile strength. However, drug release analysis indicated that the SC release from the sample can last up to 72 h. Animal experimentation confirmed that DS wound dressing positively accelerated wound closure and collagen deposition in the Wistar rat skin wound model. [Display omitted]
doi_str_mv 10.1016/j.ijbiomac.2024.131859
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source Elsevier
subjects 3D-printing
animal experimentation
Animals
Bandages
cell adhesion
chitosan
Chitosan - chemistry
Chitosan - pharmacology
citrates
collagen
Drug Liberation
drugs
electron microscopy
Electrospinning
Male
Membranes, Artificial
Polyesters - chemistry
polyvinyl alcohol
Polyvinyl Alcohol - chemistry
Printing, Three-Dimensional
Rats
Sildenafil Citrate - chemistry
Sildenafil Citrate - pharmacology
Tensile Strength
therapeutics
Tissue Scaffolds - chemistry
Wound healing
Wound Healing - drug effects
title An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing
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