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An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing
Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the fre...
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Published in: | International journal of biological macromolecules 2024-06, Vol.269 (Pt 2), p.131859-131859, Article 131859 |
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creator | Rezaei, Elham Salar Poursamar, Seyed Ali Naeimi, Mitra Taheri, Mohammad Mahdi Rafienia, Mohammad |
description | Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the frequency of dressing changes. Moreover, the outer layer is a mechanical support for the wound, preventing undue tension in the affected area. A 3D-printed polycaprolactone (PCL) membrane was utilized as the outer layer to fabricate DS wound dressing. Simultaneously, a polyvinyl alcohol/chitosan/sildenafil citrate (PVA/CS/SC) scaffold was electrospun onto the PCL membrane to facilitate cellular adhesion and proliferation. Scanning electron microscopy (SEM) analysis of the PCL filaments revealed a consistent cross-sectional surface and structure, with an average diameter of 562.72 ± 29.15 μm. SEM results also demonstrated uniform morphology and beadless structure for the PVA/CS/SC scaffold, with an average fiber diameter of 366.77 ± 1.81 nm for PVA/CS. The addition of SC led to an increase in fiber diameter while resulting in a reduction in tensile strength. However, drug release analysis indicated that the SC release from the sample can last up to 72 h. Animal experimentation confirmed that DS wound dressing positively accelerated wound closure and collagen deposition in the Wistar rat skin wound model.
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doi_str_mv | 10.1016/j.ijbiomac.2024.131859 |
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[Display omitted]</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.131859</identifier><identifier>PMID: 38728875</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>3D-printing ; animal experimentation ; Animals ; Bandages ; cell adhesion ; chitosan ; Chitosan - chemistry ; Chitosan - pharmacology ; citrates ; collagen ; Drug Liberation ; drugs ; electron microscopy ; Electrospinning ; Male ; Membranes, Artificial ; Polyesters - chemistry ; polyvinyl alcohol ; Polyvinyl Alcohol - chemistry ; Printing, Three-Dimensional ; Rats ; Sildenafil Citrate - chemistry ; Sildenafil Citrate - pharmacology ; Tensile Strength ; therapeutics ; Tissue Scaffolds - chemistry ; Wound healing ; Wound Healing - drug effects</subject><ispartof>International journal of biological macromolecules, 2024-06, Vol.269 (Pt 2), p.131859-131859, Article 131859</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c348t-2dec2305549455af554afeffbeca1d25bbe8f03489ad340000e9b3481fcb5c3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38728875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rezaei, Elham Salar</creatorcontrib><creatorcontrib>Poursamar, Seyed Ali</creatorcontrib><creatorcontrib>Naeimi, Mitra</creatorcontrib><creatorcontrib>Taheri, Mohammad Mahdi</creatorcontrib><creatorcontrib>Rafienia, Mohammad</creatorcontrib><title>An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the frequency of dressing changes. Moreover, the outer layer is a mechanical support for the wound, preventing undue tension in the affected area. A 3D-printed polycaprolactone (PCL) membrane was utilized as the outer layer to fabricate DS wound dressing. Simultaneously, a polyvinyl alcohol/chitosan/sildenafil citrate (PVA/CS/SC) scaffold was electrospun onto the PCL membrane to facilitate cellular adhesion and proliferation. Scanning electron microscopy (SEM) analysis of the PCL filaments revealed a consistent cross-sectional surface and structure, with an average diameter of 562.72 ± 29.15 μm. SEM results also demonstrated uniform morphology and beadless structure for the PVA/CS/SC scaffold, with an average fiber diameter of 366.77 ± 1.81 nm for PVA/CS. The addition of SC led to an increase in fiber diameter while resulting in a reduction in tensile strength. However, drug release analysis indicated that the SC release from the sample can last up to 72 h. Animal experimentation confirmed that DS wound dressing positively accelerated wound closure and collagen deposition in the Wistar rat skin wound model.
[Display omitted]</description><subject>3D-printing</subject><subject>animal experimentation</subject><subject>Animals</subject><subject>Bandages</subject><subject>cell adhesion</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>citrates</subject><subject>collagen</subject><subject>Drug Liberation</subject><subject>drugs</subject><subject>electron microscopy</subject><subject>Electrospinning</subject><subject>Male</subject><subject>Membranes, Artificial</subject><subject>Polyesters - chemistry</subject><subject>polyvinyl alcohol</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Printing, Three-Dimensional</subject><subject>Rats</subject><subject>Sildenafil Citrate - chemistry</subject><subject>Sildenafil Citrate - pharmacology</subject><subject>Tensile Strength</subject><subject>therapeutics</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU1vGyEQhlHVqnHS_oWIYy9rw7LY7K1Rmn5IkXppz2gWhgaLBRd2Xe0vyt8srpNec2JmeOZD70vINWdrzvh2s1_7_eDTCGbdsrZbc8GV7F-RFVe7vmGMiddkxXjHG8UFuyCXpexrdSu5eksuhNq1Su3kijzeROojPfopJwrRnpNjomWa7UKToxjQ1M9ymCM9pLAcfVwChWDSQwob8-CnVCBuig8WIzgfqKnDYEI6wkRTpOJTc8g-Tmj_9Rs45BTATClWBMchQw2gUKA2zUNAGmDBTP-kuZ5jM5bi46935I2DUPD903tFfn6--3H7tbn__uXb7c19Y0Snpqa1aFrBpOz6TkpwNQCHzg1ogNtWDgMqxyragxVdFYRhP9SUOzNII1BckQ_nufXI3zOWSY--GAyhHpnmogWXQvaq7eTLKJOi38l2e0K3Z9RUIUtGp6siI-RFc6ZPfuq9fvZTn_zUZz9r4_XTjnkY0f5vezawAh_PAFZRjh6zLsZjNGh9rr5pm_xLO_4CV0a42w</recordid><startdate>202406</startdate><enddate>202406</enddate><creator>Rezaei, Elham Salar</creator><creator>Poursamar, Seyed Ali</creator><creator>Naeimi, Mitra</creator><creator>Taheri, Mohammad Mahdi</creator><creator>Rafienia, Mohammad</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202406</creationdate><title>An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing</title><author>Rezaei, Elham Salar ; Poursamar, Seyed Ali ; Naeimi, Mitra ; Taheri, Mohammad Mahdi ; Rafienia, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-2dec2305549455af554afeffbeca1d25bbe8f03489ad340000e9b3481fcb5c3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>3D-printing</topic><topic>animal experimentation</topic><topic>Animals</topic><topic>Bandages</topic><topic>cell adhesion</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>citrates</topic><topic>collagen</topic><topic>Drug Liberation</topic><topic>drugs</topic><topic>electron microscopy</topic><topic>Electrospinning</topic><topic>Male</topic><topic>Membranes, Artificial</topic><topic>Polyesters - chemistry</topic><topic>polyvinyl alcohol</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Printing, Three-Dimensional</topic><topic>Rats</topic><topic>Sildenafil Citrate - chemistry</topic><topic>Sildenafil Citrate - pharmacology</topic><topic>Tensile Strength</topic><topic>therapeutics</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rezaei, Elham Salar</creatorcontrib><creatorcontrib>Poursamar, Seyed Ali</creatorcontrib><creatorcontrib>Naeimi, Mitra</creatorcontrib><creatorcontrib>Taheri, Mohammad Mahdi</creatorcontrib><creatorcontrib>Rafienia, Mohammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rezaei, Elham Salar</au><au>Poursamar, Seyed Ali</au><au>Naeimi, Mitra</au><au>Taheri, Mohammad Mahdi</au><au>Rafienia, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-06</date><risdate>2024</risdate><volume>269</volume><issue>Pt 2</issue><spage>131859</spage><epage>131859</epage><pages>131859-131859</pages><artnum>131859</artnum><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Double-layer dermal substitutes (DS) generally provide more effective therapeutic outcomes than single-layer substitutes. The architectural design of DS incorporates an outer layer to protect against bacterial invasions and maintain wound hydration, thereby reducing the risk of infection and the frequency of dressing changes. Moreover, the outer layer is a mechanical support for the wound, preventing undue tension in the affected area. A 3D-printed polycaprolactone (PCL) membrane was utilized as the outer layer to fabricate DS wound dressing. Simultaneously, a polyvinyl alcohol/chitosan/sildenafil citrate (PVA/CS/SC) scaffold was electrospun onto the PCL membrane to facilitate cellular adhesion and proliferation. Scanning electron microscopy (SEM) analysis of the PCL filaments revealed a consistent cross-sectional surface and structure, with an average diameter of 562.72 ± 29.15 μm. SEM results also demonstrated uniform morphology and beadless structure for the PVA/CS/SC scaffold, with an average fiber diameter of 366.77 ± 1.81 nm for PVA/CS. The addition of SC led to an increase in fiber diameter while resulting in a reduction in tensile strength. However, drug release analysis indicated that the SC release from the sample can last up to 72 h. Animal experimentation confirmed that DS wound dressing positively accelerated wound closure and collagen deposition in the Wistar rat skin wound model.
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subjects | 3D-printing animal experimentation Animals Bandages cell adhesion chitosan Chitosan - chemistry Chitosan - pharmacology citrates collagen Drug Liberation drugs electron microscopy Electrospinning Male Membranes, Artificial Polyesters - chemistry polyvinyl alcohol Polyvinyl Alcohol - chemistry Printing, Three-Dimensional Rats Sildenafil Citrate - chemistry Sildenafil Citrate - pharmacology Tensile Strength therapeutics Tissue Scaffolds - chemistry Wound healing Wound Healing - drug effects |
title | An in vitro and in vivo study of electrospun polyvinyl alcohol/chitosan/sildenafil citrate mat on 3D-printed polycaprolactone membrane as a double layer wound dressing |
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