Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava

• Published in: International journal of molecular sciences 2024-05, Vol.25 (9), p.4711
• Main Authors: Lu, Xiao-Hua, Wang, Ya-Jie, Zhen, Xing-Hou, Yu, Hui, Pan, Mu, Fu, Dong-Qing, Li, Rui-Mei, Liu, Jiao, Luo, Hai-Yan, Hu, Xin-Wen, Yao, Yuan, Guo, Jian-Chun
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - metabolism; Abscisic Acid - pharmacology; Cassava; Chloroplasts; Cloning; CRISPR; Enzymes; Ethylenes - metabolism; Flowers & plants; Gene expression; Gene Expression Regulation, Plant; Genetic engineering; Glucose; Hormones; Localization; Manihot - genetics; Manihot - metabolism; Mutation; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Promoter Regions, Genetic; Proteins; Rice; Starch Synthase - genetics; Starch Synthase - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25094711

Soluble starch synthases (SSs) play important roles in the synthesis of cassava starch. However, the expression characteristics of the cassava SSs genes have not been elucidated. In this study, the gene and its promoter, from SC8 cassava cultivars, were respectively isolated by PCR amplification. MeSSIII-1 protein was localized to the chloroplasts. qRT-PCR analysis revealed that the gene was expressed in almost all tissues tested, and the expression in mature leaves was 18.9 times more than that in tuber roots. expression was induced by methyljasmonate (MeJA), abscisic acid (ABA), and ethylene (ET) hormones in cassava. expression patterns were further confirmed in transgenic cassava. The promoter deletion analysis showed that the -264 bp to -1 bp promoter has basal activity. The range from -1228 bp to -987 bp and -488 bp to -264 bp significantly enhance promoter activity. The regions from -987 bp to -747 bp and -747 bp to -488 bp have repressive activity. These findings will provide an important reference for research on the potential function and transcriptional regulation mechanisms of the gene and for further in-depth exploration of the regulatory network of its internal functional elements.