Modular probe integrating with quantum dots based versatile platform for sensitive and label-free biomarker detection

• Published in: Talanta (Oxford) 2024-08, Vol.276, p.126228, Article 126228
• Main Authors: Wang, Huajun, Wu, Xueda, Ma, Qianli, Li, Jiayang, Fu, Bingbing, An, Jinghui
• Format: Article
• Language: English
• Subjects: Biomarkers - analysis; Biosensing Techniques - methods; C-Reactive Protein - analysis; CRP; Dual-loop probe; Exonuclease-iii; Humans; Limit of Detection; MicroRNAs - analysis; miRNA; Quantum dots (QDs); Quantum Dots - chemistry
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2024.126228

Multiplexed analysis of biomarkers in a single sample tube is essential for accurate diagnosis and therapy of diseases. However, the existing detection platforms suffer from many drawbacks, such as low specificity, limited applicable sceneries, and complicated operation. Hence, it is highly important to develop a versatile biomarker detection platform that can be used for disease diagnosis and pathophysiological research. In this study, we provide a versatile method for detecting biomarkers using dual-loop probes and quantum dots (QDs). This approach utilizes a dual-loop probe that consists of a recognition module for identifying specific targets, a template recognition module for initiating subsequent chain replacement cycles, and a signal module for facilitating the fixation of QDs on the 96-well plate. The lower limit of detection for miRNA-21 is determined to be at the aM level. Furthermore, this design may be easily expanded to simultaneously detect several targets, such as miRNA and C-reactive protein. The experimental results demonstrated the successful construction of the versatile biomarkers detection platform, and indicated that the sensitive and versatile platform has significant potential in the areas of bio-sensing, clinical diagnostics, and environmental sample analysis. [Display omitted] •We provide a versatile platform for miRNA and CRP detection that is both sensitive and portable.•The platform can be adapted to detect different biomarkers by replacing the recognition module.•The method achieves high detection sensitivity through the strand replacement cycle facilitated by DNA polymerase and endonuclease, as well as the signal cycle mediated by Exo-iii.•A detection limit as low as aM levels for the miRNA-21 is obtained.