Hydrazo Pyrazole‐Pyridone Fluorescent tag for NLO, Live cell imaging, LFPs visualization, Photophysical probing, and Electrochemical sensor for Dopamine detection

• Published in: Luminescence (Chichester, England) England), 2024-05, Vol.39 (5), p.e4760-n/a
• Main Authors: Krishnamurthy, Chethan, Jathi, Keshavayya, K. M., Pallavi, Yesudhasan, Chinnaraj
• Format: Article
• Language: English
• Subjects: azo colourant; Chemical sensors; computational study; Density Functional Theory; Dopamine; Dopamine - analysis; Dopamine - chemistry; dopamine sensing; Electrochemical Techniques; Electrochemistry; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Glassy carbon; Humans; latent fingerprints; live cell imaging; Mass spectrometry; Mass spectroscopy; Medical imaging; Molecular Structure; NMR; Nuclear magnetic resonance; Optical Imaging; Photochemical Processes; Pyrazole; Pyrazoles; Pyrazoles - chemistry; Pyridones - chemistry; Quantum chemistry; Sensitivity; Ultraviolet radiation; Voltammetry; Wavelength
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.4760

The present communication reports on the synthesis of a novel methyl‐pyridone azo fluorescent tag (MPAFT) were proven through 1H (NMR), FT‐IR, UV–vis, and high‐resolution mass spectrometry. The quantum chemical parameters of MPAFT were evaluated using density functional theory (DFT) analysis. It was further investigated for its latent fingerprint (LFPs) in various surfaces and anticounterfeiting applications. By exposing Level I–Level III, ridge features to UV light with a wavelength of 365 nm, a bioimaging investigation has also demonstrated the potential of MPAFT's emission behaviour. The cyclic voltammetry (CV) and linear sweep voltammetry (LSV) at MPAFT/MGCE (modified glassy carbon electrode) were used to explore the electrochemical sensitivity and reliable detection of dopamine (DA) in neutral PBS (pH 7) electrolyte solution, and the results show good sensitivity and detection. The lower detection limit for LSV was 0.81 μM under optimum conditions. Through UV absorption measurements, the stable state of the synthesized mono‐azo molecular characteristics of hydrazo form (–N–NH–) was established. Predicting the global reactivity characteristics involved using the molecular electrostatic potential and DFT computational studies. Using the modified glassy carbon electrode (MPAFT/MGCE) for cyclic voltammetry (CV) and linear sweep voltammetry (LSV), the electrochemical sensitivity and accurate detection of dopamine (DA) were investigated. Its latent fingerprint (LFPs) in diverse surfaces and anticounterfeiting applications were further investigated.