In-vitro and in-vivo antidiabetic activity of aerial parts of Aitchisonia rosea supported by phytochemical and GC-MS analysis

• Published in: Pakistan journal of pharmaceutical sciences 2024-01, Vol.37 (1), p.163-171
• Main Authors: Waheed, Imran, Ul Haq, Muhammad Israr, Rasool, Shahid, Javaid, Madiha, Shah, Arshad Ali, Aamir, Khurram, Ur Rehman, Muhammad Khalil, Ur Rehman, Muhammad Haseeb
• Format: Article
• Language: English
• Subjects: Alloxan; alpha-Amylases - antagonists & inhibitors; alpha-Amylases - metabolism; Animals; Blood Glucose - drug effects; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - drug therapy; Gas Chromatography-Mass Spectrometry; Glucose Tolerance Test; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Male; Phytochemicals - analysis; Phytochemicals - pharmacology; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rats; Rats, Wistar
• ISSN: 1011-601X
• DOI: 10.36721/PJPS.2024.37.1.REG.163-171.1

Medicinal plants contain a wide variety of bioactive phytoconstituents which can serve as new therapeutic agents for several diseases. This study examines the antidiabetic potential of Aitchisonia rosea in alloxan-induced diabetic rats and identifies its bioactive phytoconstituents using GC-MS. In vitro, antidiabetic potential was established using the α-amylase inhibition assay. In vivo, antidiabetic potential was investigated by employing the oral glucose tolerance test (OGTT). GC-MS analysis was used to identify the bioactive phytoconstituents. The in vitro and in vivo tests showed that the aqueous extract of A. rosea possesses better antidiabetic potential. The α-amylase inhibition assay highlighted an IC value of 134.87µg/ml. In an oral glucose tolerance test, rats given an aqueous A. rosea extract significantly lowered their blood sugar levels significant reduction in the blood glucose concentration was observed in the oral glucose tolerance test in rats treated with the aqueous A. rosea extract. GC-MS investigation revealed many phytoconstituents, with serverogenin acetate and cycloheptasiloxane tetradecamethyl being important antidiabetic agents. This study found anti-diabetic properties in A. rosea extract. The phytochemical and GC-MS investigation also found serverogenin acetate and cycloheptasiloxane tetradecamethyl, which could be used to develop new antidiabetic drugs.