The mitochondrial uncoupler 2,4-dinitrophenol modulates inflammatory and oxidative responses in Trypanosoma cruzi-induced acute myocarditis in mice

• Published in: Cardiovascular pathology 2024-09, Vol.72, p.107653, Article 107653
• Main Authors: Caetano-da-Silva, José Edson, Gonçalves-Santos, Elda, Domingues, Elisa L.B.C., Caldas, Ivo S., Lima, Graziela D.A., Diniz, Lívia F., Gonçalves, Reggiani V., Novaes, Rômulo D.
• Format: Article
• Language: English
• Subjects: Cardiovascular pathology; Chagas disease; Infectious myocarditis; Oxidative stress
• ISSN: 1054-8807; 1879-1336; 1879-1336
• DOI: 10.1016/j.carpath.2024.107653

•A 2,4-Dinitrophenol (DNP) effect was investigated in T. cruzi-induced myocarditis.•DNP was compared to Benznidazole (Bz) effect in T. cruzi-induced myocarditis.•DNP and Bz attenuated cardiac oxidative stress and inflammation in infected mice.•DNP but not Bz inhibited cardiac defenses in T. cruzi-infected mice.•DNP aggravated and Bz protected against cardiac infection in mice. By uncoupling oxidative phosphorylation, 2,4-dinitrophenol (DNP) attenuates reactive oxygen species (ROS) biosynthesis, which are known to aggravate infectious myocarditis in Chagas disease. Thus, the impact of DNP-based chemotherapy on Trypanosoma cruzi-induced acute myocarditis was investigated. C56BL/6 mice uninfected and infected untreated and treated daily with 100 mg/kg benznidazole (Bz, reference drug), 5 and 10 mg/kg DNP by gavage for 11 days after confirmation of T. cruzi infection were investigated. Twenty-four hours ​after the last treatment, the animals were euthanized and the heart was collected for microstructural, immunological and biochemical analyses. T. cruzi inoculation induced systemic inflammation (e.g., cytokines and anti-T. cruzi IgG upregulation), cardiac infection (T. cruzi DNA), oxidative stress, inflammatory infiltrate and microstructural myocardial damage in untreated mice. DNP treatment aggravated heart infection and microstructural damage, which were markedly attenuated by Bz. DNP (10 mg/kg) was also effective in attenuating ROS (total ROS, H2O2, and O2−), nitric oxide (NO), lipid (malondialdehyde - MDA) and protein (protein carbonyl - PCn) oxidation, TNF, IFN-γ, IL-10, and MCP-1/CCL2, anti-T. cruzi IgG, cardiac troponin I levels, as well as inflammatory infiltrate and cardiac damage in T. cruzi-infected mice. Our findings indicate that DNP aggravated heart infection and microstructural cardiomyocytes damage in infected mice. These responses were related to the antioxidant and anti-inflammatory properties of DNP, which favors infection by weakening the pro-oxidant and pro-inflammatory protective mechanisms of the infected host. Conversely, Bz-induced cardioprotective effects combined effective anti-inflammatory and antiparasitic responses, which protect against heart infection, oxidative stress, and microstructural damage in Chagas disease.