A validated high-performance liquid chromatography method for detecting geniposide, ellagic acid, piperine, costunolide and dehydrocostus lactone in Liuwei Muxiang capsules

In this study, a sensitive high-performance liquid chromatography detector was established and validated for the simultaneous determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. The analysis was achieved on CHANIN 100-5-C18-H column (...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2024-01, Vol.37 (1(Special)), p.205-213
Main Authors: Mao, Lei, Jiang, Lingying, Zhao, Hangyu, Lin, Jie, Huang, Lihong, Liu, Zebei, Cheng, Qunan, Dai, Xiaoyan, Wang, Xiaofen
Format: Article
Language:English
Subjects:
Alkaloids - analysis
Benzodioxoles - analysis
Capsules
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - chemistry
Ellagic Acid - analysis
Iridoids - analysis
Lactones - analysis
Piperidines - analysis
Piperidines - chemistry
Polyunsaturated Alkamides - analysis
Reproducibility of Results
Sesquiterpenes - analysis
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Summary:In this study, a sensitive high-performance liquid chromatography detector was established and validated for the simultaneous determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. The analysis was achieved on CHANIN 100-5-C18-H column (5μm, 250 mm×4.6 mm) with the temperature of 30 C. Gradient elution was applied using 0.1% phosphoric acid solution-methanol-acetonitrile (50:50) as mobile phase at the flow rate of 1.0 mL/min. The determination was performed at the wavelength of 225 nm (detecting geniposide), 254 nm (detecting ellagic acid), 343 nm (detecting piperine) and 225 nm (detecting costunolide and dehydrocostuslactone) along with the sample volume of 10μL. The linear ranges of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone demonstrated good linear relationships within their respective determination ranges. The average recoveries were 100.04%, 99.86%, 99.79%, 100.17% and 100.41%, respectively. RSD% was 1.3%, 1.2%, 1.2%, 1.2%, 1.5%, respectively. The developed method was proved to be simple, accurate and sensitive, which can provide a quantitative analysis method for the content determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang capsules.
ISSN:1011-601X