Effect of Lycium barbarum polysaccharide on osteoblast proliferation and differentiation in postmenopausal osteoporosis

• Published in: International journal of biological macromolecules 2024-06, Vol.271 (Pt 1), p.132415, Article 132415
• Main Authors: Li, Zi-Xiang, Zhuo, Jia-Lu, Yang, Ning, Gao, Ming-Bo, Qu, Zhi-Hua, Han, Ting
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - metabolism; Animals; Cell Differentiation - drug effects; Cell Line; Cell Proliferation - drug effects; Drugs, Chinese Herbal - pharmacology; Fatty Acids, Volatile - metabolism; Female; Gastrointestinal Microbiome - drug effects; Humans; Lycium barbarum polysaccharide (LBP); Mice; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteocalcin - metabolism; Osteogenesis - drug effects; Osteoporosis, Postmenopausal - drug therapy; Osteoporosis, Postmenopausal - metabolism; Postmenopausal osteoporosis (PMO); Pre-osteoblasts of mouse calvaria (MC3T3-E1); Proliferation and differentiation
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132415

We aimed to investigate the effect of Lycium barbarum polysaccharide (LBP) on the proliferation and differentiation of osteoblasts in postmenopausal individuals with osteoporosis using in vitro cell experiments. We assessed the effect of long-term LBP consumption on the intestinal metabolites of individuals using a simulation of the human intestinal microbiota ecosystem. We also tested the capacity of LBP in proliferating MC3T3-E1 cells using the cell counting kit-8 (CCK-8) method and analyzed the effect of intestinal metabolites on the osteogenic differentiation of MC3T3-E1 cells by testing bone metabolism viability with relevant indicators. The level of short-chain fatty acids (SCFAs) significantly increased (p