A case control study investigating the methylation levels of GHRL and GHSR genes in alcohol use disorder

Background Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to...

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Bibliographic Details
Published in:Molecular biology reports 2024-12, Vol.51 (1), p.663-663, Article 663
Main Authors: Özkan-Kotiloğlu, Selin, Kaya-Akyüzlü, Dilek, Güven, Emine, Doğan, Özlem, Ağtaş-Ertan, Ece, Özgür-İlhan, İnci
Format: Article
Language:English
Subjects:
Addictions
Addictive behaviors
Adult
Alcohol use
Alcoholism - genetics
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Case-Control Studies
DNA methylation
DNA Methylation - genetics
Female
Ghrelin
Ghrelin - blood
Ghrelin - genetics
Histology
Humans
Life Sciences
Male
Middle Aged
Morphology
Original Article
Polymorphism, Single Nucleotide - genetics
Receptors, Ghrelin - genetics
Restriction fragment length polymorphism
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Summary:Background Alcohol use disorder (AUD) is a relapsing disease described as excessive use of alcohol. Evidence of the role of DNA methylation in addiction is accumulating. Ghrelin is an important peptide known as appetite hormone and its role in addictive behavior has been identified. Here we aimed to determine the methylation levels of two crucial genes ( GHRL and GHSR ) in ghrelin signaling and further investigate the association between methylation ratios and plasma ghrelin levels. Methods Individuals diagnosed with ( n  = 71) and without ( n  = 82) AUD were recruited in this study. DNA methylation levels were measured through methylation-sensitive high-resolution melting (MS-HRM). Acylated ghrelin levels were detected by ELISA. The GHRL rs696217 polymorphism was analyzed by the standard PCR-RFLP method. Results GHRL was significantly hypermethylated ( P  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-024-09585-4