Application of Cystoseira myrica phycosynthesized selenium nanoparticles incorporated with nano‐chitosan to control aflatoxigenic fungi in fish feed

• Published in: Journal of the science of food and agriculture 2024-09, Vol.104 (12), p.7678-7687
• Main Authors: Tayel, Ahmed A, El‐madawy, Karima M, Moussa, Shaaban H, Assas, Mona A
• Format: Article
• Language: English
• Subjects: Algae; antifungal action; Antifungal activity; Antifungal agents; application; Aspergillus flavus; biosynthesis; Chitosan; Conjugation; Cystoseira; Fish; Fish feeds; Fluconazole; Food contamination; Food sources; Fungi; Fungicides; Infrared analysis; Nanocomposites; Nanomaterials; Nanoparticles; Nanotechnology; Qualitative analysis; Selenium; X-ray diffraction
• ISSN: 0022-5142; 1097-0010; 1097-0010
• DOI: 10.1002/jsfa.13604

BACKGROUND The recurrent contaminations of feed materials with mycotoxigenic fungi can endanger both farmed animals and humans. Biosynthesized nanomaterials are assumingly the ideal agents to overcome fungal invasion in feed/foodstuffs, especially when utilizing sustainable sources for synthesis. Herein, the phycosynthesis of selenium nanoparticles (SeNPs) was targeted using Cystoseira myrica algal extract (CE), and the conjugation of CE/SeNPs with chitosan nanoparticles (NCt) to produce potential antifungal nanocomposites for controlling Aspergillus flavus isolates in fish feed. RESULTS The phycosynthesis of SeNPs with CE was effectually carried out and validated using visible/UV analysis, X‐ray diffraction and transmission microscopy; CE/SeNPs had diameters of 8.7 nm and spherical shapes. NCt/CE/SeNPs nanocomposite (173.3 nm mean diameter) was achieved and the component interactions were validated via infrared spectroscopic analysis. The antifungal assessment of screened nanomaterials against three Aspergillus flavus strains indicated that NCt/CE/SeNPs exceeded the fluconazole action using qualitative/quantitative assays. Severe alteration/distortions in A. flavus mycelial structure and morphology were microscopically observed within 48 h of NCt/CE/SeNPs treatment. The treatment of feed ingredients (crushed corn and feed powder) by blending with nanomaterials (NCt, CE/SeNPs and NCt/CE/SeNPs) led to significant reduction in A. flavus count/growth after storage for 7 days; NCt/CE/SeNPs could completely inhibit any fungal growth in feed material. CONCLUSION The pioneering phycosynthesis of CE/SeNPs and their nanoconjugation with NCt generated bioactive antifungal agents to control A. flavus strains. The innovatively constructed NCt/CE/SeNPs nanocomposite is advised for application as an effectual, biosafe and natural fungicidal conjugate for the protection of fish feed from mycotoxigenic fungi. © 2024 Society of Chemical Industry.