Biosorption of chromium by live and dead cells of Bacillus nitratireducens isolated from textile effluent

• Published in: Chemosphere (Oxford) 2024-07, Vol.359, p.142389-142389, Article 142389
• Main Authors: Imron, Muhammad Fauzul, Setiawan, Wahyu, Putranto, Trisnadi Widyaleksono Catur, Abdullah, Siti Rozaimah Sheikh, Kurniawan, Setyo Budi
• Format: Article
• Language: English
• Subjects: Adsorption; Bacillus - metabolism; Bacillus nitratireducens; Bioaccumulation; Biodegradation, Environmental; Biosorption; Chromium - metabolism; Dead cells; Heavy metals; Spectroscopy, Fourier Transform Infrared; Textile Industry; Textiles; Waste Disposal, Fluid - methods; Wastewater - chemistry; Wastewater - microbiology; Wastewater treatment; Water Pollutants, Chemical - metabolism
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.142389

Bacillus nitratireducens was isolated from textile effluent and showed high tolerance to chromium (Cr), reaching up to a 1000 mg/L MIC value. This research was aimed at utilizing biosorbents from live and dead cells of B. nitratireducens to remove Cr from an aqueous solution. A batch biosorption test was performed, and mechanisms analysis was approached by an adsorption-desorption test, SEM-EDS, and FTIR analysis. Cr removal by dead cells in 25, 50, and 100 mg/L of Cr were 58.99 ± 0.7%, 69.8 ± 0.2%, and 82.87 ± 0.11%, respectively, while that by live cells was 73.08 ± 1.9%, 80.27 ± 6.33%, and 86.17 ± 1.93%, respectively. Live cells showed significantly higher Cr removal and adsorption capacities as compared to dead cells. In all concentrations, absorption contributed more than adsorption to the Cr removal by both live and dead cells. Absorption of Cr was subjected to occur due to passive mechanisms in dead cells while involving some active mechanisms in live cells. SEM-EDS confirmed the detection of Cr on the cell surface, while FTIR revealed the shifting of some peaks after the biosorption test, suggesting interactions between Cr and functional groups. Further TEM analysis is suggested to be conducted as a future approach to reveal the inner structure of cells and confirm the involvement of absorption mechanisms. [Display omitted] •Live and dead cells of Bacillus nitratireducens performed biosorption of Cr.•Live cells showed higher adsorption capacity.•Active and passive mechanisms suggested to occur in live cells.•Absorption > adsorption for both biosorbents.•Maximum Cr removal >80% for both biosorbents.