Tedizolid phosphate alleviates DSS-induced ulcerative colitis by inhibiting senescence of cell and colon tissue through activating AMPK signaling pathway

• Published in: International immunopharmacology 2024-06, Vol.135, p.112286-112286, Article 112286
• Main Authors: Zhou, Min, Liu, Zhen-lin, Liu, Jia-yu, Wang, Xiao-bo
• Format: Article
• Language: English
• Subjects: AMP-Activated Protein Kinases - metabolism; AMPK; Animals; Cell Line; Cellular Senescence - drug effects; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - drug effects; Colon - pathology; Dextran Sulfate; Disease Models, Animal; Humans; Inflammation; Intestinal senescence; Male; Mice; Mice, Inbred C57BL; Organophosphates - pharmacology; Organophosphates - therapeutic use; Oxazolidinones - pharmacology; Oxazolidinones - therapeutic use; Signal Transduction - drug effects; Tedizolid Phosphate; Ulcerative colitis
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2024.112286

•Ulcerative colitis (UC) inducer dextran sulfate sodium (DSS) triggers aging in human colon epithelial cells and mice colon tissues.•The anti-aging drug Tedizolid Phosphate (TED) can relieve colitis caused by DSS.•TED alleviates aging caused by DSS through activating AMPK signaling pathway. Ulcerative colitis (UC) is a subtype of inflammatory bowel disease. Previous studies have suggested a link between senescence process and the body’s inflammatory reaction, indicating that senescence may exacerbate UC, yet the relation between UC and senescence remains unclear. Tedizolid Phosphate (TED), a novel oxazolidinone antimicrobial, is indicated in acute bacterial skin infections, its impact on senescence is not known. Our research revealed that the UC inducer dextran sulfate sodium (DSS) triggers senescence in both colon epithelial NCM460 cells and colon tissues, and TED that screened from a compound library demonstrated a strong anti-senescence effect on DSS treated NCM460 cells. As an anti-senescence medication identified in this research, TED efficiently alleviated UC and colonic senescence in mice caused by DSS. By proteomic analysis and experimental validation, we found that DSS significantly inhibits the AMPK signaling pathway, while TED counteracts senescence by restoring AMPK activity. This research verified that the development of UC is accompanied with colon tissue senescence, and TED, an anti-senescence medication, can effectively treat UC caused by DSS and alleviate colon senescence. Our work suggests anti-senescence strategy is an effective approach for UC treatment.