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The Role of microRNA in the Regulation of Cortisol Metabolism in the Adipose Tissue in the Course of Obesity
The similarity of the clinical picture of metabolic syndrome and hypercortisolemia supports the hypothesis that obesity may be associated with impaired expression of genes related to cortisol action and metabolism in adipose tissue. The expression of genes encoding the glucocorticoid receptor alpha...
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| Published in: | International journal of molecular sciences 2024-05, Vol.25 (10), p.5058 |
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| container_issue | 10 |
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| container_title | International journal of molecular sciences |
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| creator | Podraza, Jakub Gutowska, Klaudia Lenartowicz, Anna Wąsowski, Michał Jonas, Marta Izabela Bartoszewicz, Zbigniew Lisik, Wojciech Jonas, Maurycy Binda, Artur Jaworski, Paweł Tarnowski, Wiesław Noszczyk, Bartłomiej Puzianowska-Kuźnicka, Monika Kuryłowicz, Alina |
| description | The similarity of the clinical picture of metabolic syndrome and hypercortisolemia supports the hypothesis that obesity may be associated with impaired expression of genes related to cortisol action and metabolism in adipose tissue. The expression of genes encoding the glucocorticoid receptor alpha (
), cortisol metabolizing enzymes (
,
,
), and adipokines, as well as selected microRNAs, was measured by real-time PCR in adipose tissue from 75 patients with obesity, 19 patients following metabolic surgery, and 25 normal-weight subjects. Cortisol levels were analyzed by LC-MS/MS in 30 pairs of tissues. The mRNA levels of all genes studied were significantly (
< 0.05) decreased in the visceral adipose tissue (VAT) of patients with obesity and normalized by weight loss. In the subcutaneous adipose tissue (SAT),
and
were affected by this phenomenon. Negative correlations were observed between the mRNA levels of the investigated genes and selected miRNAs (hsa-miR-142-3p, hsa-miR-561, and hsa-miR-579). However, the observed changes did not translate into differences in tissue cortisol concentrations, although levels of this hormone in the SAT of patients with obesity correlated negatively with mRNA levels for adiponectin. In conclusion, although the expression of genes related to cortisol action and metabolism in adipose tissue is altered in obesity and miRNAs may be involved in this process, these changes do not affect tissue cortisol concentrations. |
| doi_str_mv | 10.3390/ijms25105058 |
| format | article |
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), cortisol metabolizing enzymes (
,
,
), and adipokines, as well as selected microRNAs, was measured by real-time PCR in adipose tissue from 75 patients with obesity, 19 patients following metabolic surgery, and 25 normal-weight subjects. Cortisol levels were analyzed by LC-MS/MS in 30 pairs of tissues. The mRNA levels of all genes studied were significantly (
< 0.05) decreased in the visceral adipose tissue (VAT) of patients with obesity and normalized by weight loss. In the subcutaneous adipose tissue (SAT),
and
were affected by this phenomenon. Negative correlations were observed between the mRNA levels of the investigated genes and selected miRNAs (hsa-miR-142-3p, hsa-miR-561, and hsa-miR-579). However, the observed changes did not translate into differences in tissue cortisol concentrations, although levels of this hormone in the SAT of patients with obesity correlated negatively with mRNA levels for adiponectin. In conclusion, although the expression of genes related to cortisol action and metabolism in adipose tissue is altered in obesity and miRNAs may be involved in this process, these changes do not affect tissue cortisol concentrations.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms25105058</identifier><identifier>PMID: 38791098</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics ; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism ; 11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics ; 11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism ; Adipocytes ; Adipose Tissue - metabolism ; Adipose tissues ; Adult ; Androgens ; Body fat ; Carbohydrate Dehydrogenases ; Corticosteroids ; Dehydrogenases ; Diabetes ; Enzymes ; Female ; Gene expression ; Gene Expression Regulation ; Genes ; Hormones ; Humans ; Hydrocortisone - metabolism ; Insulin resistance ; Intra-Abdominal Fat - metabolism ; Male ; Metabolism ; Metabolites ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; Obesity ; Obesity - genetics ; Obesity - metabolism ; Physiological aspects ; Receptors, Glucocorticoid - genetics ; Receptors, Glucocorticoid - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Steroids ; Type 2 diabetes ; Weight control</subject><ispartof>International journal of molecular sciences, 2024-05, Vol.25 (10), p.5058</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c381t-797f66bb8101dc1567663305ff3e4709634c83271c88f6f771553c4fc9bd630e3</cites><orcidid>0009-0001-7278-6386 ; 0000-0003-3020-3979 ; 0000-0001-8632-4927 ; 0000-0003-3707-0419 ; 0000-0001-5295-3848</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3059424014/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3059424014?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,25730,27900,27901,36988,36989,44565,75095</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38791098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Podraza, Jakub</creatorcontrib><creatorcontrib>Gutowska, Klaudia</creatorcontrib><creatorcontrib>Lenartowicz, Anna</creatorcontrib><creatorcontrib>Wąsowski, Michał</creatorcontrib><creatorcontrib>Jonas, Marta Izabela</creatorcontrib><creatorcontrib>Bartoszewicz, Zbigniew</creatorcontrib><creatorcontrib>Lisik, Wojciech</creatorcontrib><creatorcontrib>Jonas, Maurycy</creatorcontrib><creatorcontrib>Binda, Artur</creatorcontrib><creatorcontrib>Jaworski, Paweł</creatorcontrib><creatorcontrib>Tarnowski, Wiesław</creatorcontrib><creatorcontrib>Noszczyk, Bartłomiej</creatorcontrib><creatorcontrib>Puzianowska-Kuźnicka, Monika</creatorcontrib><creatorcontrib>Kuryłowicz, Alina</creatorcontrib><title>The Role of microRNA in the Regulation of Cortisol Metabolism in the Adipose Tissue in the Course of Obesity</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The similarity of the clinical picture of metabolic syndrome and hypercortisolemia supports the hypothesis that obesity may be associated with impaired expression of genes related to cortisol action and metabolism in adipose tissue. The expression of genes encoding the glucocorticoid receptor alpha (
), cortisol metabolizing enzymes (
,
,
), and adipokines, as well as selected microRNAs, was measured by real-time PCR in adipose tissue from 75 patients with obesity, 19 patients following metabolic surgery, and 25 normal-weight subjects. Cortisol levels were analyzed by LC-MS/MS in 30 pairs of tissues. The mRNA levels of all genes studied were significantly (
< 0.05) decreased in the visceral adipose tissue (VAT) of patients with obesity and normalized by weight loss. In the subcutaneous adipose tissue (SAT),
and
were affected by this phenomenon. Negative correlations were observed between the mRNA levels of the investigated genes and selected miRNAs (hsa-miR-142-3p, hsa-miR-561, and hsa-miR-579). However, the observed changes did not translate into differences in tissue cortisol concentrations, although levels of this hormone in the SAT of patients with obesity correlated negatively with mRNA levels for adiponectin. In conclusion, although the expression of genes related to cortisol action and metabolism in adipose tissue is altered in obesity and miRNAs may be involved in this process, these changes do not affect tissue cortisol concentrations.</description><subject>11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics</subject><subject>11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism</subject><subject>11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics</subject><subject>11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism</subject><subject>Adipocytes</subject><subject>Adipose Tissue - metabolism</subject><subject>Adipose tissues</subject><subject>Adult</subject><subject>Androgens</subject><subject>Body fat</subject><subject>Carbohydrate Dehydrogenases</subject><subject>Corticosteroids</subject><subject>Dehydrogenases</subject><subject>Diabetes</subject><subject>Enzymes</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Hormones</subject><subject>Humans</subject><subject>Hydrocortisone - metabolism</subject><subject>Insulin resistance</subject><subject>Intra-Abdominal Fat - metabolism</subject><subject>Male</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>Obesity</subject><subject>Obesity - genetics</subject><subject>Obesity - metabolism</subject><subject>Physiological aspects</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>Receptors, Glucocorticoid - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Steroids</subject><subject>Type 2 diabetes</subject><subject>Weight control</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNptkc1P3DAQxa2qqFDaW88oUi8cWBhnYjs-rlZAK_EhrbbnKHHG1CsnXuLkwH-Pt7DtFiEfbD3_5ulpHmPfOJwjarhw6y7mgoMAUX5gR7zI8xmAVB_33ofsc4xrgBxzoT-xQyyV5qDLI-ZXvylbBk9ZsFnnzBCWd_PM9dm41elh8vXoQr_9XYRhdDH47JbGugnexW4Hzlu3CZGylYtxop26CNMQ_xjfNxTd-PSFHdjaR_r6eh-zX1eXq8WP2c399c_F_GZmsOTjTGllpWyakgNvDRdSSYkIwlqkQoGWWJgSc8VNWVppleJCoCms0U0rEQiP2emL72YIjxPFsepcNOR93VOYYoUgARVK0An9_gZdp9R9SpcooYu8AF78ox5qT5XrbRiH2mxNq7nSAnXBc0zU-TtUOi2lzYaerEv6fwNnLwNp7TEOZKvN4Lp6eKo4VNtyq_1yE37ymnVqOmr_wrs28RmthZvU</recordid><startdate>20240507</startdate><enddate>20240507</enddate><creator>Podraza, Jakub</creator><creator>Gutowska, Klaudia</creator><creator>Lenartowicz, Anna</creator><creator>Wąsowski, Michał</creator><creator>Jonas, Marta Izabela</creator><creator>Bartoszewicz, Zbigniew</creator><creator>Lisik, Wojciech</creator><creator>Jonas, Maurycy</creator><creator>Binda, Artur</creator><creator>Jaworski, Paweł</creator><creator>Tarnowski, Wiesław</creator><creator>Noszczyk, Bartłomiej</creator><creator>Puzianowska-Kuźnicka, Monika</creator><creator>Kuryłowicz, Alina</creator><general>MDPI AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0001-7278-6386</orcidid><orcidid>https://orcid.org/0000-0003-3020-3979</orcidid><orcidid>https://orcid.org/0000-0001-8632-4927</orcidid><orcidid>https://orcid.org/0000-0003-3707-0419</orcidid><orcidid>https://orcid.org/0000-0001-5295-3848</orcidid></search><sort><creationdate>20240507</creationdate><title>The Role of microRNA in the Regulation of Cortisol Metabolism in the Adipose Tissue in the Course of Obesity</title><author>Podraza, Jakub ; Gutowska, Klaudia ; Lenartowicz, Anna ; Wąsowski, Michał ; Jonas, Marta Izabela ; Bartoszewicz, Zbigniew ; Lisik, Wojciech ; Jonas, Maurycy ; Binda, Artur ; Jaworski, Paweł ; Tarnowski, Wiesław ; Noszczyk, Bartłomiej ; Puzianowska-Kuźnicka, Monika ; Kuryłowicz, Alina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-797f66bb8101dc1567663305ff3e4709634c83271c88f6f771553c4fc9bd630e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics</topic><topic>11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism</topic><topic>11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics</topic><topic>11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism</topic><topic>Adipocytes</topic><topic>Adipose Tissue - metabolism</topic><topic>Adipose tissues</topic><topic>Adult</topic><topic>Androgens</topic><topic>Body fat</topic><topic>Carbohydrate Dehydrogenases</topic><topic>Corticosteroids</topic><topic>Dehydrogenases</topic><topic>Diabetes</topic><topic>Enzymes</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Hormones</topic><topic>Humans</topic><topic>Hydrocortisone - metabolism</topic><topic>Insulin resistance</topic><topic>Intra-Abdominal Fat - metabolism</topic><topic>Male</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>Obesity</topic><topic>Obesity - 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Academic</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Podraza, Jakub</au><au>Gutowska, Klaudia</au><au>Lenartowicz, Anna</au><au>Wąsowski, Michał</au><au>Jonas, Marta Izabela</au><au>Bartoszewicz, Zbigniew</au><au>Lisik, Wojciech</au><au>Jonas, Maurycy</au><au>Binda, Artur</au><au>Jaworski, Paweł</au><au>Tarnowski, Wiesław</au><au>Noszczyk, Bartłomiej</au><au>Puzianowska-Kuźnicka, Monika</au><au>Kuryłowicz, Alina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of microRNA in the Regulation of Cortisol Metabolism in the Adipose Tissue in the Course of Obesity</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2024-05-07</date><risdate>2024</risdate><volume>25</volume><issue>10</issue><spage>5058</spage><pages>5058-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>The similarity of the clinical picture of metabolic syndrome and hypercortisolemia supports the hypothesis that obesity may be associated with impaired expression of genes related to cortisol action and metabolism in adipose tissue. The expression of genes encoding the glucocorticoid receptor alpha (
), cortisol metabolizing enzymes (
,
,
), and adipokines, as well as selected microRNAs, was measured by real-time PCR in adipose tissue from 75 patients with obesity, 19 patients following metabolic surgery, and 25 normal-weight subjects. Cortisol levels were analyzed by LC-MS/MS in 30 pairs of tissues. The mRNA levels of all genes studied were significantly (
< 0.05) decreased in the visceral adipose tissue (VAT) of patients with obesity and normalized by weight loss. In the subcutaneous adipose tissue (SAT),
and
were affected by this phenomenon. Negative correlations were observed between the mRNA levels of the investigated genes and selected miRNAs (hsa-miR-142-3p, hsa-miR-561, and hsa-miR-579). However, the observed changes did not translate into differences in tissue cortisol concentrations, although levels of this hormone in the SAT of patients with obesity correlated negatively with mRNA levels for adiponectin. In conclusion, although the expression of genes related to cortisol action and metabolism in adipose tissue is altered in obesity and miRNAs may be involved in this process, these changes do not affect tissue cortisol concentrations.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38791098</pmid><doi>10.3390/ijms25105058</doi><orcidid>https://orcid.org/0009-0001-7278-6386</orcidid><orcidid>https://orcid.org/0000-0003-3020-3979</orcidid><orcidid>https://orcid.org/0000-0001-8632-4927</orcidid><orcidid>https://orcid.org/0000-0003-3707-0419</orcidid><orcidid>https://orcid.org/0000-0001-5295-3848</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | PMC (PubMed Central); Publicly Available Content (ProQuest) |
| subjects | 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism 11-beta-Hydroxysteroid Dehydrogenase Type 2 - genetics 11-beta-Hydroxysteroid Dehydrogenase Type 2 - metabolism Adipocytes Adipose Tissue - metabolism Adipose tissues Adult Androgens Body fat Carbohydrate Dehydrogenases Corticosteroids Dehydrogenases Diabetes Enzymes Female Gene expression Gene Expression Regulation Genes Hormones Humans Hydrocortisone - metabolism Insulin resistance Intra-Abdominal Fat - metabolism Male Metabolism Metabolites MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism Middle Aged Obesity Obesity - genetics Obesity - metabolism Physiological aspects Receptors, Glucocorticoid - genetics Receptors, Glucocorticoid - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Steroids Type 2 diabetes Weight control |
| title | The Role of microRNA in the Regulation of Cortisol Metabolism in the Adipose Tissue in the Course of Obesity |
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