Benzoyl Chloride Derivatization Advances the Quantification of Dissolved Polar Metabolites on Coral Reefs

• Published in: Journal of proteome research 2024-06, Vol.23 (6), p.2041-2053
• Main Authors: Garcia, Brianna M., Becker, Cynthia C., Weber, Laura, Swarr, Gretchen J., Kido Soule, Melissa C., Apprill, Amy, Kujawinski, Elizabeth B.
• Format: Article
• Language: English
• Subjects: Animals; Anthozoa - chemistry; Anthozoa - metabolism; Carbon - chemistry; Carbon - metabolism; Coral Reefs; Ecosystem; Mass Spectrometry - methods; Metabolome; Metabolomics - methods; United States Virgin Islands
• ISSN: 1535-3893; 1535-3907; 1535-3907
• DOI: 10.1021/acs.jproteome.4c00049

Extracellular chemical cues constitute much of the language of life among marine organisms, from microbes to mammals. Changes in this chemical pool serve as invisible signals of overall ecosystem health and disruption to this finely tuned equilibrium. In coral reefs, the scope and magnitude of the chemicals involved in maintaining reef equilibria are largely unknown. Processes involving small, polar molecules, which form the majority components of labile dissolved organic carbon, are often poorly captured using traditional techniques. We employed chemical derivatization with mass spectrometry-based targeted exometabolomics to quantify polar dissolved phase metabolites on five coral reefs in the U.S. Virgin Islands. We quantified 45 polar exometabolites, demonstrated their spatial variability, and contextualized these findings in terms of geographic and benthic cover differences. By comparing our results to previously published coral reef exometabolomes, we show the novel quantification of 23 metabolites, including central carbon metabolism compounds (e.g., glutamate) and novel metabolites such as homoserine betaine. We highlight the immense potential of chemical derivatization-based exometabolomics for quantifying labile chemical cues on coral reefs and measuring molecular level responses to environmental stressors. Overall, improving our understanding of the composition and dynamics of reef exometabolites is vital for effective ecosystem monitoring and management strategies.