Influence of different presentation forms of chlorhexidine on contaminated root canals during agitation

• Published in: Australian endodontic journal 2024-12, Vol.50 (3), p.513-524
• Main Authors: Lopes, Ana B. S., R. Lima, Augusto, D. Bronzato, Juliana, Herrera, Daniel R., Francisco, Priscila A., Carvalho, Maria C. C., Abuna, Gabriel, Sinhoreti, Mario, Gomes, Brenda P. F. A.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents, Local - pharmacology; Anti-Infective Agents, Local - therapeutic use; Bicuspid; Cell activation; Chlorhexidine; Chlorhexidine - pharmacology; Chlorhexidine - therapeutic use; Colony Count, Microbial; Confocal microscopy; Decontamination; Dental Pulp Cavity - microbiology; Dentin; Dentin - drug effects; Dentin - microbiology; Disinfection - methods; endodontics; Enterococcus faecalis - drug effects; Gels; Humans; Lavage; microbiology; Microscopy, Confocal; root canal irrigants; Root Canal Irrigants - pharmacology; Root Canal Irrigants - therapeutic use; Root Canal Preparation - instrumentation; Root Canal Preparation - methods; Root canals; Sonication - methods; Therapeutic Irrigation - methods
• ISSN: 1329-1947; 1747-4477; 1747-4477
• DOI: 10.1111/aej.12862

This in vitro study aimed to verify the influence of chlorhexidine (CHX) (gel and solution) in association with different activation protocols on disinfection of root canals contaminated with Enterococcus faecalis. In total, 120 lower premolar roots were selected, contaminated and divided into 12 groups according to irrigation technique and substance. Samples were collected before and after each irrigation technique and analysed for colony‐forming units (CFU). Three dentin discs were obtained for quantification of viable intratubular cells by using confocal laser scanning microscopy (CLSM). CFU results showed that sonic and ultrasonic were more effective than conventional irrigation. Ultrasonic activation was more effective than sonic for CHX gel (p