Sensitive colorimetric detection of Vibrio vulnificus based on target-induced shielding against the peroxidase-mimicking activity of CeO2@PtRu nanozyme

Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO2@PtRu with high per...

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Bibliographic Details
Published in:Food chemistry 2024-10, Vol.454, p.139757, Article 139757
Main Authors: Xu, Chenjing, Xie, Jinpo, Yu, Lian, Shu, Bin, Liu, Xiaogang, Chen, Siping, Li, Qinglan, Qi, Shaohai, Zhao, Suqing
Format: Article
Language:English
Subjects:
Biotin-streptavidin system
CeO2@PtRu nanozyme
Colorimetric biosensor
Vibrio vulnificus
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Summary:Vibrio vulnificus infection caused by contaminated aquatic products and seawater can lead to severe disease and high mortality. The development of a rapid and sensitive detection method for Vibrio vulnificus is vital to effectively prevent infection in advance. In this study, CeO2@PtRu with high peroxidase activity was used to construct a colorimetric immunoassay for Vibrio vulnificus detection by conjugating polyclonal antibodies via the biotin-streptavidin system. The developed colorimetric biosensor for Vibrio vulnificus demonstrated rapid operability and good sensitivity with a detection range from 104 CFU/mL to 109 CFU/mL, and the limit of detection (LOD) is 193 CFU/mL. Moreover, the colorimetric biosensor showed excellent specificity and good recoveries from 98.70% to 102.10% with RSD 
ISSN:0308-8146
1873-7072
1873-7072
DOI:10.1016/j.foodchem.2024.139757