Silencing the fatty acid elongase gene elovl6 induces reprogramming of nutrient metabolism in male Oreochromis niloticus

• Published in: International journal of biological macromolecules 2024-06, Vol.271 (Pt 1), p.132666, Article 132666
• Main Authors: Tao, Yi-Fan, Pan, Yi-Fan, Zhong, Chun-Yi, Wang, Qing-Chun, Hua, Ji-Xiang, Lu, Si-Qi, Li, Yan, Dong, Ya-Lun, Xu, Pao, Jiang, Bing-Jie, Qiang, Jun
• Format: Article
• Language: English
• Subjects: Acetyltransferases - genetics; Acetyltransferases - metabolism; Amino Acid Sequence; Animals; Antisense RNA; Cichlids - genetics; Cichlids - metabolism; Cloning, Molecular; elovl6; Fatty Acid Elongases - genetics; Fatty Acid Elongases - metabolism; Fatty Acids - metabolism; Gene Expression Regulation; Gene Knockdown Techniques; Gene Silencing; Lipid Metabolism - genetics; Liver - metabolism; Male; Nutrient metabolism; Nutrients - metabolism; Oreochromis niloticus; Regulatory mechanism
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132666

Elongation of very long-chain fatty acids protein 6 (ELOVL6) plays a pivotal role in the synthesis of endogenous fatty acids, influencing energy balance and metabolic diseases. The primary objective of this study was to discover the molecular attributes and regulatory roles of ELOVL6 in male Nile tilapia, Oreochromis niloticus. The full-length cDNA of elovl6 was cloned from male Nile tilapia, and was determined to be 2255-bp long, including a 5′-untranslated region of 193 bp, a 3′-untranslated region of 1252 bp, and an open reading frame of 810 bp encoding 269 amino acids. The putative protein had typical features of ELOVL proteins. The transcript levels of elovl6 differed among various tissues and among fish fed with different dietary lipid sources. Knockdown of elovl6 in Nile tilapia using antisense RNA technology resulted in significant alterations in hepatic morphology, long-chain fatty acid synthesis, and fatty acid oxidation, and led to increased fat deposition in the liver and disrupted glucose/lipid metabolism. A comparative transcriptomic analysis (elovl6 knockdown vs. the negative control) identified 5877 differentially expressed genes with significant involvement in key signaling pathways including the peroxisome proliferator-activated receptor signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and the insulin signaling pathway, all of which are crucial for lipid and glucose metabolism. qRT-PCR analyses verified the transcript levels of 13 differentially expressed genes within these pathways. Our findings indicate that elovl6 knockdown in male tilapia impedes oleic acid synthesis, culminating in aberrant nutrient metabolism. [Display omitted] •Nile tilapia elovl6 encodes a protein with typical characteristics of ELOVL proteins.•The highest transcript level of elovl6 was in the liver.•The transcript level of elovl6 was significantly influenced by dietary lipid sources.•elovl6 knockdown impedes oleic acid synthesis, culminating in aberrant glucose/lipid metabolism.