Sugarcane ScCAX4 is a Negative Regulator of Resistance to Pathogen Infection

• Published in: Journal of agricultural and food chemistry 2024-06, Vol.72 (23), p.13205-13216
• Main Authors: Zhang, Chang, Li, Zhenxiang, Sun, Tingting, Zang, Shoujian, Wang, Dongjiao, Su, Yachun, Wu, Qibin, Que, Youxiong
• Format: Article
• Language: English
• Subjects: Biotechnology and Biological Transformations; Cyclopentanes - metabolism; Disease Resistance - genetics; Ethylenes - metabolism; Fusarium - physiology; Gene Expression Regulation, Plant; Nicotiana - genetics; Nicotiana - immunology; Nicotiana - metabolism; Nicotiana - microbiology; Oxylipins - metabolism; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - immunology; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - immunology; Plants, Genetically Modified - metabolism; Plants, Genetically Modified - microbiology; Saccharum - genetics; Saccharum - immunology; Saccharum - metabolism; Saccharum - microbiology; Salicylic Acid - metabolism
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c00805

Calcium (Ca2+) is a second messenger in various physiological processes within plants. The significance of the Ca2+/H+ exchanger (CAX) has been established in facilitating Ca2+ transport in plants; however, disease resistance functions of the CAX gene remain elusive. In this study, we conducted sequence characterization and expression analysis for a sugarcane CAX gene, ScCAX4 (GenBank Accession Number: MW206380). In order to further investigate the disease resistance functions, this gene was then transiently overexpressed in Nicotiana benthamiana leaves, which were subsequently inoculated with Fusarium solani var. coeruleum. Results showed that ScCAX4 overexpression increased the susceptibility of N. benthamiana to pathogen infection by regulating the expression of genes related to salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) pathways, suggesting its negative role in disease resistance. Furthermore, we genetically transformed the ScCAX4 gene into N. benthamiana and obtained three positive T2 generation lines. Interestingly, the symptomatology of transgenic plants was consistent with that of transient overexpression after pathogen inoculation. Notably, the JA content in transgenic overexpression lines was significantly higher than that in the wild-type. RNA-seq revealed that ScCAX4 could mediate multiple signaling pathways, and the JA signaling pathway played a key role in modulating disease resistance. Finally, a regulatory model was depicted for the increased susceptibility to pathogen infection conferred by the ScCAX4 gene. This study provides genetic resources for sugarcane molecular breeding and the research direction for plant CAX genes.