Optimal and sustainable production of tailored fish protein hydrolysates from tuna canning wastes and discarded blue whiting: Effect of protein molecular weight on chemical and bioactive properties

• Published in: The Science of the total environment 2024-08, Vol.939, p.173461-173461, Article 173461
• Main Authors: Vázquez, José Antonio, Comesaña, Sara, Soengas, José Luis, Pérez, Montse, Bermúdez, Roberto, Rotllant, Josep, Valcarcel, Jesus
• Format: Article
• Language: English
• Subjects: Animals; Bioactives; Blue whiting discards; Fish protein hydrolysates; Fish Proteins; Gadiformes; Mathematical modeling; Molecular Weight; Protein Hydrolysates; Protein molecular weight; Sustainability; Tuna; Tuna by-products
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.173461

Thousands tons of discards of blue whiting (BW) and tuna heads (YT) by-products are generated each year in Europe. BW is the species most discarded by European fishing fleet and, in some canning factories, YT are processed for the retrieval of oil rich in omega-3, but producing a huge amount of solid remains and effluents disposal as wastes. The development of optimal and sustainable processes for both substrates is mandatory in order to reach clean solutions under the circular economy precepts. This work focused on the mathematical optimization of the production of tailored fish protein hydrolysates (FPH), from blue whiting and tuna residues, in terms of controlling average molecular weights (Mw) of proteins. For the modeling of the protein depolymerization time-course, a pseudo-mechanistic model was used, which combined a reaction mechanistic equation affected, in the kinetic parameters, by two non-lineal equations (a first-order kinetic and like-Weibull formulae). In all situations, experimental data were accurately simulated by that model achieving R2 values higher than 0.96. The validity of the experimental conditions obtained from modeling were confirmed performing productions of FPH at scale of 5 L-reactor, without pH-control in most of cases, at the different ranges of Mw selected (1–2 kDa, 2–5 kDa and 5–10 kDa). The results showed that FPH from BW with lower Mw led to a remarkable yield of production (12 % w/w of substrate), largest protein contents (77 % w/w of BW hydrolysate), greatest in vitro digestibility (>95 %), highest essential amino acid presence (43 %) and the best antioxidant (DPPH = 62 %) and antihypertensive (IC50-ACE = 80 mg/L) properties. Our results prove that the proposed procedure to produce sustainable FPH, with specific Mw characterisitics, could be extended to other fish waste substrates. Tailored FPH may have the potential to serve as valuable ingredients for functional foods and high-quality aquaculture feed. [Display omitted] •Sustainable valorization of fishing discards and canning wastes.•Mathematical modeling of protein molecular weight dynamics on enzyme hydrolysis.•Tailored protein hydrolysates produced at different molecular weight intervals.•Smaller protein hydrolysates led to higher production yields and bioactives.•This process may contribute to the sustainability of the fish industry.